A surrogate cell‐based SARS‐CoV‐2 spike blocking assay

Abstract: To monitor infection by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and successful vaccination against coronavirus disease 2019 (COVID-19), the kinetics of neutralizing or blocking anti-SARS-CoV-2 antibody titers need to be assessed. Here, we report the development of a quick and inexpensive surrogate SARS-CoV-2 blocking assay (SUBA) using immobilized recombinant human angiotensin-converting enzyme 2 (hACE2) and human cells expressing the native form of surface SARS-CoV-2 spike protein. Spike protein-expressing cells bound to hACE2 in the absence or presence of blocking antibodies were quantified by measuring the optical density of cell-associated crystal violet in a spectrophotometer. The advantages are that SUBA is a fast and inexpensive assay, which does not require biosafety level 2- or 3-approved laboratories. Most importantly, SUBA detects blocking antibodies against the native trimeric cell-bound SARS-CoV-2 spike protein and can be rapidly adjusted to quickly pre-screen already approved therapeutic antibodies or sera from vaccinated individuals for their ACE2 blocking activities against any emerging SARS-CoV-2 variants

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: European journal of immunology. - 51, 11 (2021) , 2665-2676, ISSN: 1521-4141

Classification and Topics

• Classification: Medizin, Gesundheit

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2024
• Creator: Schuh, Wolfgang ; Baus, Lena ; Steinmetz, Tobit ; Schulz, Sebastian R. ; Weckwerth, Leonie ; Roth, Edith ; Hauke, Manuela ; Krause, Sara ; Morhart, Patrick Daniel ; Rauh, Manfred ; Hoffmann, Markus ; Vesper, Niklas ; Reth, Michael ; Schneider, Holm ; Jäck, Hans-Martin ; Mielenz, Dirk Alexander

Further information

• DOI: 10.1002/eji.202149302
• URN: urn:nbn:de:bsz:25-freidok-2482323
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:54 PM CET