A physcomitrella PIN protein acts in spermatogenesis and sporophyte retention
Abstract: The auxin efflux PIN-FORMED (PIN) proteins are conserved in all land plants and important players in plant development. In the moss Physcomitrella (Physcomitrium patens), three canonical PINs (PpPINA–C) are expressed in the leafy shoot (gametophore). PpPINA and PpPINB show functional activity in vegetative growth and sporophyte development. Here, we examined the role of PpPINC in the life cycle of Physcomitrella.
We established reporter and knockout lines for PpPINC and analysed vegetative and reproductive tissues using microscopy and transcriptomic sequencing of moss gametangia.
PpPINC is expressed in immature leaves, mature gametangia and during sporophyte development. The sperm cells (spermatozoids) of pinC knockout mutants exhibit increased motility and an altered flagella phenotype. Furthermore, the pinC mutants have a higher portion of differentially expressed genes related to spermatogenesis, increased fertility and an increased abortion rate of premeiotic sporophytes.
Here, we show that PpPINC is important for spermatogenesis and sporophyte retention. We propose an evolutionary conserved way of polar growth during early moss embryo development and sporophyte attachment to the gametophore while suggesting the mechanical function in sporophyte retention of a ring structure, the Lorch ring
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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New phytologist. - 237, 6 (2023) , 2118-2135, ISSN: 1469-8137
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2023
- Creator
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Lüth, Volker
Rempfer, Christine
Gessel, Nico van
Herzog, Oliver
Hanser, Melanie
Braun, Marion
Decker, Eva
Reski, Ralf
- DOI
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10.1111/nph.18691
- URN
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urn:nbn:de:bsz:25-freidok-2337692
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:52 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Lüth, Volker
- Rempfer, Christine
- Gessel, Nico van
- Herzog, Oliver
- Hanser, Melanie
- Braun, Marion
- Decker, Eva
- Reski, Ralf
- Universität
Time of origin
- 2023
Other Objects (12)
Zell- und molekularbiologische Untersuchungen der Cytokinin-induzierbaren Gewebedifferenzierung und Chloroplastenteilung bei Physcomitrella patens (Hedw.) B.S.G.
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Quantitative Analyse des auxininduzierten miR160/ARF-Regelkreises in Physcomitrella patens
The homeobox gene BELL1 is the master regulator for the developmental switch from gametophyte to sporophyte in Physcomitrella patens
Spindle motility skews division site determination during asymmetric cell division in Physcomitrella
Protein encoding genes in an ancient plant: analysis of codon usage, retained genes and splice sites in a moss, Physcomitrella patens
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Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: a review
Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella
Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells
Zell- und molekularbiologische Untersuchungen der Cytokinin-induzierbaren Gewebedifferenzierung und Chloroplastenteilung bei Physcomitrella patens (Hedw.) B.S.G.
Microscopy of Physcomitrella patens sperm cells
Quantitative Analyse des auxininduzierten miR160/ARF-Regelkreises in Physcomitrella patens
The homeobox gene BELL1 is the master regulator for the developmental switch from gametophyte to sporophyte in Physcomitrella patens
Spindle motility skews division site determination during asymmetric cell division in Physcomitrella
Protein encoding genes in an ancient plant: analysis of codon usage, retained genes and splice sites in a moss, Physcomitrella patens
The moss bioreactor offers best of both worlds for biopharmaceutical production
Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: a review
Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella
Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells
Zell- und molekularbiologische Untersuchungen der Cytokinin-induzierbaren Gewebedifferenzierung und Chloroplastenteilung bei Physcomitrella patens (Hedw.) B.S.G.
Microscopy of Physcomitrella patens sperm cells
Quantitative Analyse des auxininduzierten miR160/ARF-Regelkreises in Physcomitrella patens
The homeobox gene BELL1 is the master regulator for the developmental switch from gametophyte to sporophyte in Physcomitrella patens
Spindle motility skews division site determination during asymmetric cell division in Physcomitrella
Protein encoding genes in an ancient plant: analysis of codon usage, retained genes and splice sites in a moss, Physcomitrella patens
The moss bioreactor offers best of both worlds for biopharmaceutical production
Automated and semi-automated enhancement, segmentation and tracing of cytoskeletal networks in microscopic images: a review
Viral suppressor of RNA silencing in vascular plants also interferes with the development of the bryophyte Physcomitrella patens
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude
Multifactorial analysis of terminator performance on heterologous gene expression in Physcomitrella