A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
Abstract: Cross‐linking mass spectrometry (XL‐MS) is an attractive method for the proteome‐wide characterization of protein structures and interactions. Currently, the depth of in vivo XL‐MS studies is lagging behind the established applications to cell lysates, because cross‐linking reagents that can penetrate intact cells and strategies to enrich cross‐linked peptides lack efficiency. To tackle these limitations, we have developed a phosphonate‐containing cross‐linker, tBu‐PhoX, that efficiently permeates various biological membranes and can be robustly enriched using routine immobilized metal ion affinity chromatography. We have established a tBu‐PhoX‐based in vivo XL‐MS approach that enables cross‐links in intact human cells to be identified in high numbers with substantially reduced analysis time. Collectively, the developed cross‐linker and XL‐MS approach pave the way for the comprehensive XL‐MS characterization of living systems.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry ; day:27 ; month:01 ; year:2022 ; extent:1
Angewandte Chemie / International edition. International edition ; (27.01.2022) (gesamt 1)
- Urheber
-
Jiang, Pin‐Lian
Wang, Cong
Diehl, Anne
Viner, Rosa
Etienne, Chris
Nandhikonda, Premchendar
Foster, Leigh
Bomgarden, Ryan D.
Liu, Fan
- DOI
-
10.1002/anie.202113937
- URN
-
urn:nbn:de:101:1-2022012714194957604771
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:24 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Jiang, Pin‐Lian
- Wang, Cong
- Diehl, Anne
- Viner, Rosa
- Etienne, Chris
- Nandhikonda, Premchendar
- Foster, Leigh
- Bomgarden, Ryan D.
- Liu, Fan
Ähnliche Objekte (12)
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
Cross-linking with microbial transglutaminase
Selective cross‐linking of coinciding protein assemblies by in‐gel cross‐linking mass spectrometry
Cross-linking dots on metal oxides
The Biochemistry of Fibrin Cross-Linking
Chemical Cross-Linking of Factor VIII Subunits
Graphite nanoplatelet chemical cross-linking by elemental sulfur
PACK-CXL: Corneal cross-linking in infectious keratitis
Severe Complications after Corneal Collagen Cross-Linking (CXL)
Evaluation of Iontophoretic Collagen Cross-linking for Early Stage of Progressive Keratoconus Compared to Standard Cross-linking: A Non-Inferiority Study
Proteome-scale recombinant standards and a robust high-speed search engine to advance cross-linking MS-based interactomics
Corneal cross-linking in 9 horses with ulcerative keratitis
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
Cross-linking with microbial transglutaminase
Selective cross‐linking of coinciding protein assemblies by in‐gel cross‐linking mass spectrometry
Cross-linking dots on metal oxides
The Biochemistry of Fibrin Cross-Linking
Chemical Cross-Linking of Factor VIII Subunits
Graphite nanoplatelet chemical cross-linking by elemental sulfur
PACK-CXL: Corneal cross-linking in infectious keratitis
Severe Complications after Corneal Collagen Cross-Linking (CXL)
Evaluation of Iontophoretic Collagen Cross-linking for Early Stage of Progressive Keratoconus Compared to Standard Cross-linking: A Non-Inferiority Study
Proteome-scale recombinant standards and a robust high-speed search engine to advance cross-linking MS-based interactomics
Corneal cross-linking in 9 horses with ulcerative keratitis
A Membrane‐Permeable and Immobilized Metal Affinity Chromatography (IMAC) Enrichable Cross‐Linking Reagent to Advance In Vivo Cross‐Linking Mass Spectrometry
Cross-linking with microbial transglutaminase
Selective cross‐linking of coinciding protein assemblies by in‐gel cross‐linking mass spectrometry
Cross-linking dots on metal oxides
The Biochemistry of Fibrin Cross-Linking
Chemical Cross-Linking of Factor VIII Subunits
Graphite nanoplatelet chemical cross-linking by elemental sulfur
PACK-CXL: Corneal cross-linking in infectious keratitis
Severe Complications after Corneal Collagen Cross-Linking (CXL)
Evaluation of Iontophoretic Collagen Cross-linking for Early Stage of Progressive Keratoconus Compared to Standard Cross-linking: A Non-Inferiority Study
Proteome-scale recombinant standards and a robust high-speed search engine to advance cross-linking MS-based interactomics