Preadaptation of cell numbers for wound healing assays : A critical assessment in light of standardized requirements for biomaterials testing
Abstract: In vitro wound healing assays are a suitable application to verify the efficiency of pharmaceuticals or growth factors that will be incorporated in or immobilized to e.g. electrospun biomaterials for wound dressings or other biological devices in advance. Thereby, various factors like culture conditions or cell density influence the specific cell proliferation. Hence, to establish a wound healing assay for various cell types, a stepwise adaptation of cell numbers was done for better estimation and comparison of cell density for the validation of the influence of drugs on the wound healing process. Cell proliferation of different tissue relevant cell types was evaluated by impedance measurements and live cell imaging. Cell numbers could be successfully adapted for assay specific cell densities. In general, a universal comparison of biological or chemical materials and agents in vitro may require the creation of appropriate ISO or OECD standards for a consistent and cell specific adaptation or demand of initial cell density.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Preadaptation of cell numbers for wound healing assays ; volume:7 ; number:2 ; year:2021 ; pages:295-298 ; extent:4
Current directions in biomedical engineering ; 7, Heft 2 (2021), 295-298 (gesamt 4)
- Urheber
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Markhoff, Jana
Brietzke, Andreas
Grabow, Niels
- DOI
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10.1515/cdbme-2021-2075
- URN
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urn:nbn:de:101:1-2022101214243252950343
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:30 MESZ
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Beteiligte
- Markhoff, Jana
- Brietzke, Andreas
- Grabow, Niels
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