Protein microarray generation by in situ protein expression from template DNA
Today, whole genomes are analyzed by Next‐Generation Sequencing systems, or displayed by DNA microarray in situ synthesis. However, genomic data are mainly static and do not exactly reveal the complexity of protein interaction networks of any organism or cell. To investigate protein interactions, the generation of protein microarrays, displaying whole proteomes is a prerequisite. But traditional protein microarray generation is time consuming, costly, and is restricted by a wide range of technical difficulties concerning cell culturing, protein purification, and transfer onto microarray. Some of these obstacles can be bypassed by application of cell‐free expression systems, enabling fast in situ synthesis of protein microarrays without need for cell culturing. This review provides a historical timeline of the different methods to generate protein microarrays by cell‐free expression, highlights differences and similarities, and reports the current state of the different approaches.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Protein microarray generation by in situ protein expression from template DNA ; volume:14 ; number:4 ; year:2014 ; pages:352-364 ; extent:13
Engineering in life sciences ; 14, Heft 4 (2014), 352-364 (gesamt 13)
- Creator
- DOI
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10.1002/elsc.201300052
- URN
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urn:nbn:de:101:1-2023011907593009615864
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kilb, Normann
- Burger, Jürgen
- Roth, Günter
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Wirkung hochfrequenter elektromagnetischer Felder auf DNA, Proteine und DNA-Protein-Komplexe
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(PS)2-v2: template-based protein structure prediction server
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High quality protein microarray using in situprotein purification
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