Fluorogenic Granzyme A Substrates Enable Real‐Time Imaging of Adaptive Immune Cell Activity

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Abstract: Cytotoxic immune cells, including T lymphocytes (CTLs) and natural killer (NK) cells, are essential components of the host response against tumors. CTLs and NK cells secrete granzyme A (GzmA) upon recognition of cancer cells; however, there are very few tools that can detect physiological levels of active GzmA with high spatiotemporal resolution. Herein, we report the rational design of the near‐infrared fluorogenic substrates for human GzmA and mouse GzmA. These activity‐based probes display very high catalytic efficiency and selectivity over other granzymes, as shown in tissue lysates from wild‐type and GzmA knock‐out mice. Furthermore, we demonstrate that the probes can image how adaptive immune cells respond to antigen‐driven recognition of cancer cells in real time.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Fluorogenic Granzyme A Substrates Enable Real‐Time Imaging of Adaptive Immune Cell Activity ; day:16 ; month:01 ; year:2023 ; extent:8
Angewandte Chemie / International edition. International edition ; (16.01.2023) (gesamt 8)

Creator: Cheng, Zhiming
Thompson, Emily J.
Mendive‐Tapia, Lorena
Scott, Jamie I.
Benson, Sam
Kitamura, Takanori
Senan‐Salinas, Ana
Samarakoon, Youhani
Roberts, Edward W.
Arias, Maykel A.
Pardo, Julian
Galvez, Eva M.
Vendrell, Marc

DOI: 10.1002/anie.202216142

URN: urn:nbn:de:101:1-2023011714143438010394

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:21 AM CEST

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Associated

Cheng, Zhiming
Thompson, Emily J.
Mendive‐Tapia, Lorena
Scott, Jamie I.
Benson, Sam
Kitamura, Takanori
Senan‐Salinas, Ana
Samarakoon, Youhani
Roberts, Edward W.
Arias, Maykel A.
Pardo, Julian
Galvez, Eva M.
Vendrell, Marc

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