Purification and anticonvulsant activity of xanthotoxin (8-methoxypsoralen)
Abstract: The aim of the present study was to determine the anticonvulsant effects of xanthotoxin (8-methoxypsoralen) isolated from the fruit of Pastinaca sativa L. This plant is used in European traditional medicine, including Poland. For this purpose, high-performance counter-current chromatography was used. Different solvent systems, mixtures of n-heptane, ethyl acetate, methanol and water were tested in order to calculate partition coefficients. Finally, a mixture with the ratio of 1:1:1:1 (v/v/v/v), giving the K value = 0.92, was chosen as optimal. A rapid scale-up process from analytical to preparative was developed. Evaluation of the anticonvulsant action of xanthotoxin in the mouse maximal electroshock-induced seizure test revealed that it produced a clear-cut anticonvulsant action in mice, and the experimentally-derived median effective doses (ED50 values) ranged between 219 and 252 mg kg−1.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Purification and anticonvulsant activity of xanthotoxin (8-methoxypsoralen) ; volume:9 ; number:4 ; year:2014 ; pages:431-436 ; extent:6
Open life sciences ; 9, Heft 4 (2014), 431-436 (gesamt 6)
- Creator
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Skalicka-Woźniak, Krystyna
Zagaja, Mirosław
Głowniak, Kazimierz
Łuszczki, Jarogniew
- DOI
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10.2478/s11535-013-0275-9
- URN
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urn:nbn:de:101:1-2409211601467.040451212223
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:34 AM CEST
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Associated
- Skalicka-Woźniak, Krystyna
- Zagaja, Mirosław
- Głowniak, Kazimierz
- Łuszczki, Jarogniew
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