Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity

Abstract: The segregation of the readily releasable pool of synaptic vesicles (RRP) in sub-poolsthat are differentially poised for exocytosis shapes short-term plasticity. However, the frequency-dependent mobilization of these sub-pools is poorly understood. Using slice recordings andmodeling of synaptic activity at cerebellar granule cell to Purkinje cell synapses of mice, wedescribe two sub-pools in the RRP that can be differentially recruited upon ultrafast changes in thestimulation frequency. We show that at low-frequency stimulations, a first sub-pool is graduallysilenced, leading to full blockage of synaptic transmission. Conversely, a second pool of synapticvesicles that cannot be released by a single stimulus is recruited within milliseconds by high-frequency stimulation and support an ultrafast recovery of neurotransmitter release after low-frequency depression. This frequency-dependent mobilization or silencing of sub-pools in the RRPin terminals of granule cells may play a role in the filtering of sensorimotor information in thecerebellum

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: eLife. - 6 (2017) , e28935, ISSN: 2050-084X

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2020
• Creator: Doussau, Frédéric ; Schmidt, Hartmut ; Dorgans, Kevin ; Valera, Antoine M. ; Poulain, Bernard ; Isope, Philippe

Further information

• DOI: 10.7554/elife.28935
• URN: urn:nbn:de:bsz:25-freidok-1539813
• Rights: Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:43 PM CET