Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel
Abstract: When a tooth is diseased or damaged through caries, bioactive molecules are liberated from the pulp and dentin as part of the natural response to injury and these are key molecules for stimulating stem cell responses for tissue repair. Incorporation of these extracellular‐matrix (ECM)‐derived molecules into a hydrogel model can mimic in vivo conditions to enable dentin–pulp complex regeneration. Here, a chitosan/alginate (C/A) hydrogel is developed to sequester bovine ECM extracts. Human dental pulp cells (hDPCs) are cultured with these constructs and proliferation and cytotoxicity assays confirm that these C/A hydrogels are bioactive. Sequential z‐axis fluorescent imaging visualizes hDPCs protruding into the hydrogel as it degraded. Alizarin red S staining shows that hDPCs cultured with the hydrogels display increased calcium‐ion deposition, with dentin ECM stimulating the highest levels. Alkaline phosphatase activity is increased, as is expression of transforming growth factor‐beta as demonstrated using immunocytochemistry. Directional analysis following phase contrast kinetic image capture demonstrates that both dentin and pulp ECM molecules act as chemoattractants for hDPCs. Data from this study demonstrate that purified ECM from dental pulp and dentin when delivered in a C/A hydrogel stimulates dental tissue repair processes in vitro.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan/Alginate Hydrogel ; day:09 ; month:07 ; year:2024 ; extent:13
Macromolecular bioscience ; (09.07.2024) (gesamt 13)
- Creator
-
Gould, Maree L.
Downes, Nerida J.
Woolley, Adele G.
Hussaini, Haizal M.
Ratnayake, Jithendra T.
Ali, Mohammad Azam
Friedlander, Lara T.
Cooper, Paul R.
- DOI
-
10.1002/mabi.202400254
- URN
-
urn:nbn:de:101:1-2407101421440.165887453882
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
11.12.2025, 6:09 AM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Gould, Maree L.
- Downes, Nerida J.
- Woolley, Adele G.
- Hussaini, Haizal M.
- Ratnayake, Jithendra T.
- Ali, Mohammad Azam
- Friedlander, Lara T.
- Cooper, Paul R.
Other Objects (12)
Pulp dentin biology in restorative dentistry
CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures
Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
The dynamics of TGF-β in dental pulp, odontoblasts and dentin
Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells
Hydrogels and Dentin-Pulp Complex Regeneration : From the Benchtop to Clinical Translation
Effect of Artificial Saliva Contamination on Bond Strength to Pulp Chamber Dentin
Biocompatibility and antimicrobial effect of demineralised dentin matrix hydrogel for dental pulp preservation
Photocrosslinkable gelatin-treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration: I. synthesis, characterizations and grafting optimization
Marginal adaptation, physicochemical and rheological properties of treated dentin matrix hydrogel as a novel injectable pulp capping material for dentin regeneration
Dentin-like tissue formation and biomineralization by multicellular human pulp cell spheres in vitro
The effects and potential applications of concentrated growth factor in dentin–pulp complex regeneration
Pulp dentin biology in restorative dentistry
CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures
Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
The dynamics of TGF-β in dental pulp, odontoblasts and dentin
Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells
Hydrogels and Dentin-Pulp Complex Regeneration : From the Benchtop to Clinical Translation
Effect of Artificial Saliva Contamination on Bond Strength to Pulp Chamber Dentin
Biocompatibility and antimicrobial effect of demineralised dentin matrix hydrogel for dental pulp preservation
Photocrosslinkable gelatin-treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration: I. synthesis, characterizations and grafting optimization
Marginal adaptation, physicochemical and rheological properties of treated dentin matrix hydrogel as a novel injectable pulp capping material for dentin regeneration
Dentin-like tissue formation and biomineralization by multicellular human pulp cell spheres in vitro
The effects and potential applications of concentrated growth factor in dentin–pulp complex regeneration
Pulp dentin biology in restorative dentistry
CD146 positive human dental pulp stem cells promote regeneration of dentin/pulp-like structures
Biodegradation of an injectable treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration
The dynamics of TGF-β in dental pulp, odontoblasts and dentin
Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells
Hydrogels and Dentin-Pulp Complex Regeneration : From the Benchtop to Clinical Translation
Effect of Artificial Saliva Contamination on Bond Strength to Pulp Chamber Dentin
Biocompatibility and antimicrobial effect of demineralised dentin matrix hydrogel for dental pulp preservation
Photocrosslinkable gelatin-treated dentin matrix hydrogel as a novel pulp capping agent for dentin regeneration: I. synthesis, characterizations and grafting optimization
Marginal adaptation, physicochemical and rheological properties of treated dentin matrix hydrogel as a novel injectable pulp capping material for dentin regeneration
Dentin-like tissue formation and biomineralization by multicellular human pulp cell spheres in vitro