Precise Control of Intracellular Trafficking and Receptor‐Mediated Endocytosis in Living Cells and Behaving Animals

Abstract: Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real‐time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor‐mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking‐related disorders.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Precise Control of Intracellular Trafficking and Receptor‐Mediated Endocytosis in Living Cells and Behaving Animals ; day:14 ; month:10 ; year:2024 ; extent:15 ; Advanced science ; (14.10.2024) (gesamt 15)

Beteiligte, Orts- und Zeitangaben

• Urheber: Chen, Shiau‐Chi ; Zeng, Neng‐Jie ; Liu, Grace Y. ; Wang, Hsien‐Chu ; Lin, Tzu‐Ying ; Tai, Yi‐Ling ; Chen, Chiao‐Yun ; Fang, Yin ; Chuang, Yi‐Chien ; Kao, Ching‐Lin ; Cheng, Hsuan ; Wu, Bing‐Huang ; Sun, Pin‐Chiao ; Bayansan, Odvogmed ; Chiu, Yu‐Ting ; Shih, Chi‐Hsuan ; Chung, Wen‐Hong ; Yang, Jia‐Bin ; Wang, Lily Hui‐Ching ; Chiang, Po‐Han ; Chen, Chun‐Hao ; Wagner, Oliver I. ; Wang, Yi‐Ching ; Lin, Yu‐Chun

Weitere Informationen

• DOI: 10.1002/advs.202405568
• URN: urn:nbn:de:101:1-2410151425266.945390769698
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:38 MESZ