Generation of a gene-corrected isogenic control iPSC line from cystic fibrosis patient-specific iPSCs homozygous for p.Phe508del mutation mediated by TALENs and ssODN
Abstract: Cystic fibrosis (CF) is a monogenetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which affects multiple organs. Human induced pluripotent stem cells (iPSCs) derived from CF patients and the generation of isogeneic gene-corrected control cell lines enable disease modelling, drug discovery or toxicological studies and therefore the development of CF patient-specific therapies. We have previously generated a hiPSC line from a CF patient homozygous for the p.Phe508del mutation. Here we used TALENs and single-stranded oligonucleotides to correct the mutated triplet in our CF-iPSC line
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
Stem cell research. - 23 (2017) , 95-97, ISSN: 1876-7753
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
- Beteiligte Personen und Organisationen
- DOI
-
10.1016/j.scr.2017.07.010
- URN
-
urn:nbn:de:bsz:25-freidok-1431723
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:46 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
Entstanden
- 2023
Ähnliche Objekte (12)
Lucky iPSCs
iPSCs are safe!
Modelling urea cycle disorders using iPSCs
Microhomology-assisted scarless genome editing in human iPSCs
Modeling anthracycline-induced cardiotoxicity with patient-specific iPSCs
Generating and characterizing primate iPSCs for evolutionary analyses
An evaluation of an open access iPSC training course: “How to model interstitial lung disease using patient-derived iPSCs”
Targeted integration of inducible Caspase-9 in human iPSCs allows efficient in vitro clearance of iPSCs and iPSC-macrophages
Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs
Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs
Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs
Lucky iPSCs
iPSCs are safe!
Modelling urea cycle disorders using iPSCs
Microhomology-assisted scarless genome editing in human iPSCs
Modeling anthracycline-induced cardiotoxicity with patient-specific iPSCs
Generating and characterizing primate iPSCs for evolutionary analyses
An evaluation of an open access iPSC training course: “How to model interstitial lung disease using patient-derived iPSCs”
Targeted integration of inducible Caspase-9 in human iPSCs allows efficient in vitro clearance of iPSCs and iPSC-macrophages
Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs
Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs
Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs
Lucky iPSCs
iPSCs are safe!
Modelling urea cycle disorders using iPSCs
Microhomology-assisted scarless genome editing in human iPSCs
Modeling anthracycline-induced cardiotoxicity with patient-specific iPSCs
Generating and characterizing primate iPSCs for evolutionary analyses
An evaluation of an open access iPSC training course: “How to model interstitial lung disease using patient-derived iPSCs”
Targeted integration of inducible Caspase-9 in human iPSCs allows efficient in vitro clearance of iPSCs and iPSC-macrophages
Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs
Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs