Isolation and characterization of a novel α-amylase from a metagenomic library of Western Ghats of Kerala, India

Abstract: In the present study, metagenomic library of Western Ghats soil sample was constructed in a fosmid vector (pCC1FOS) and screened for biocatalytic properties. The clones showed amylolytic activity on Luria-Bertani starch agar plates and one of them was studied in detail. The enzyme exhibited stability at elevated temperature with 60°C being the optimal temperature. The enzyme retained more than 30% activity after 60 min incubation at 80°C. It also showed more than 70% activity retention in 1.5 M NaCl solution. The pH optimum of the enzyme was at pH = 5.0. The enzyme possesses good activity in the presence of chelating and strong reducing agents with activity enhancements or retention being observed at 5 mM β-mercaptoethanol, dithiothreitol and N-bromosuccinimide. However, almost complete loss of activity was observed with 5 mM EDTA, while activity enhancement was observed upon incubation with Ca2+ suggesting it to be a Ca2+-dependent α-amylase, which was further confirmed by a thin-layer chromatography (TLC). The TLC run revealed that digestion pattern was similar to commercial α-amylase. The 16S rRNA gene sequence (GenBank accession number HQ680979) BLAST showed 95% similarities with Exiguobacterium sp. AFB-11 and AFB 18, with query sequence coverage of 99%.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Isolation and characterization of a novel α-amylase from a metagenomic library of Western Ghats of Kerala, India ; volume:66 ; number:6 ; year:2011 ; pages:939-944 ; extent:6 ; Biologia ; 66, Heft 6 (2011), 939-944 (gesamt 6)

Beteiligte, Orts- und Zeitangaben

• Urheber: Vidya, Jalaja ; Swaroop, Sweta ; Singh, Sudheer ; Alex, Deepthy ; Sukumaran, Rajeev ; Pandey, Ashok

Weitere Informationen

• DOI: 10.2478/s11756-011-0126-y
• URN: urn:nbn:de:101:1-2409221731472.625060863637
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:24 MESZ