Multiplex SERS Phenotyping of Single Cancer Cells in Microdroplets
Abstract: In the context of personalized medicine, the analysis of single cells is key in order to understand the origin and evolution of cancer to provide an accurate prognosis. Microfluidics and microdroplets are increasingly used for the handling and understanding of the behavior of single cells, as they offer the perfect isolated environment. However, due to the small volumes handled, it is necessary to couple this technology with an ultrasensitive detection technique. Herein, surface‐enhanced Raman scattering (SERS) spectroscopy and droplet microfluidics are combined toward the multiplex phenotypic analysis of single cancer cells. For this, cancer cells are labeled with different SERS tags that recognize membrane proteins and encapsulated individually in microdroplets. Afterward, single cells within microdroplets are imaged by SERS spectroscopy. To the best of the authors’ knowledge, this is the first time that a multiplex phenotypic SERS analysis of single cells in microdroplets is shown. This integrated optofluidic platform paves the way toward the multiplex and automated characterization of cell populations in cancer patients.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Multiplex SERS Phenotyping of Single Cancer Cells in Microdroplets ; day:14 ; month:11 ; year:2022 ; extent:11
Advanced optical materials ; (14.11.2022) (gesamt 11)
- Creator
-
Oliveira, Kevin
Teixeira, Alexandra
Fernandes, José M.
Lopes, Cláudia
Chícharo, Alexandre
Piairo, Paulina
Wu, Lei
Rodríguez‐Lorenzo, Laura
Diéguez, Lorena
Abalde‐Cela, Sara
- DOI
-
10.1002/adom.202201500
- URN
-
urn:nbn:de:101:1-2022111414133030484099
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Oliveira, Kevin
- Teixeira, Alexandra
- Fernandes, José M.
- Lopes, Cláudia
- Chícharo, Alexandre
- Piairo, Paulina
- Wu, Lei
- Rodríguez‐Lorenzo, Laura
- Diéguez, Lorena
- Abalde‐Cela, Sara
Other Objects (12)
In Situ Activation of Snap‐Through Instability in Multi‐Response Metamaterials through Multistable Topological Transformation
Enhanced thermal oxidative stability of silicone rubber by using cerium-ferric complex oxide as thermal oxidative stabilizer
On the accuracy of macroscopic equations for linearized rarefied gas flows
On the coverage of neutralino dark matter in coannihilations at the upgraded LHC
Zero modes activation to reconcile floppiness, rigidity, and multistability into an all-in-one class of reprogrammable metamaterials
An indirect probe of the higgsino world at the CEPC
Multiscale simulation of rarefied gas dynamics via direct intermittent GSIS-DSMC coupling
Impact of high-speed railway network on county-level accessibility and economic linkage in Jiangxi Province, China: A spatio-temporal data analysis
Lightning Data Analysis of the CMA Network in China
Application of a modified distributed-dynamic erosion and sediment yield model in a typical watershed of a hilly and gully region, Chinese Loess Plateau
Anomalous top-Higgs couplings and top polarisation in single top and Higgs associated production at the LHC
IDEF method-based simulation model design and development framework
In Situ Activation of Snap‐Through Instability in Multi‐Response Metamaterials through Multistable Topological Transformation
Enhanced thermal oxidative stability of silicone rubber by using cerium-ferric complex oxide as thermal oxidative stabilizer
On the accuracy of macroscopic equations for linearized rarefied gas flows
On the coverage of neutralino dark matter in coannihilations at the upgraded LHC
Zero modes activation to reconcile floppiness, rigidity, and multistability into an all-in-one class of reprogrammable metamaterials
An indirect probe of the higgsino world at the CEPC
Multiscale simulation of rarefied gas dynamics via direct intermittent GSIS-DSMC coupling
Impact of high-speed railway network on county-level accessibility and economic linkage in Jiangxi Province, China: A spatio-temporal data analysis
Lightning Data Analysis of the CMA Network in China
Application of a modified distributed-dynamic erosion and sediment yield model in a typical watershed of a hilly and gully region, Chinese Loess Plateau
Anomalous top-Higgs couplings and top polarisation in single top and Higgs associated production at the LHC
IDEF method-based simulation model design and development framework
In Situ Activation of Snap‐Through Instability in Multi‐Response Metamaterials through Multistable Topological Transformation
Enhanced thermal oxidative stability of silicone rubber by using cerium-ferric complex oxide as thermal oxidative stabilizer
On the accuracy of macroscopic equations for linearized rarefied gas flows
On the coverage of neutralino dark matter in coannihilations at the upgraded LHC
Zero modes activation to reconcile floppiness, rigidity, and multistability into an all-in-one class of reprogrammable metamaterials
An indirect probe of the higgsino world at the CEPC
Multiscale simulation of rarefied gas dynamics via direct intermittent GSIS-DSMC coupling
Impact of high-speed railway network on county-level accessibility and economic linkage in Jiangxi Province, China: A spatio-temporal data analysis
Lightning Data Analysis of the CMA Network in China
Application of a modified distributed-dynamic erosion and sediment yield model in a typical watershed of a hilly and gully region, Chinese Loess Plateau
Anomalous top-Higgs couplings and top polarisation in single top and Higgs associated production at the LHC