Rotational alignment of corneal endothelial grafts and risk of graft detachment after Descemet membrane endothelial keratoplasty: a double‐masked pseudo‐randomized study

Abstract: Purpose
The posterior cornea is rotationally asymmetric, and Descemet membrane endothelial keratoplasty (DMEK) grafts preferentially scroll vertically. This prospective study assessed whether graft attachment after DMEK differed depending on the rotational alignment of the donor graft in the recipient eye.

Methods
Pseudo-randomization and blinding of the graft orientation in the recipient’s eye were possible by procedural separation: (1) The eye bank recorded the position of an orientation marker in the donor cornea; (2) the surgeon preparing the DMEK graft recorded an upside-down marker relative to the eye bank marker; and (3) the surgeon assessed the position of the upside-down marker in the recipient after DMEK. Surgeons were masked towards the eye bank marker. Using mixed-effects models, we assessed graft attachment relative to the rotational alignment of the donor graft.

Results
Postoperatively, the graft was not fully attached in 59 of 179 eyes (33%). A second air fill (rebubbling) was performed in 11%. The graft axis was in line with the recipient cornea axis in 40%, oblique in 28% and orthogonal in 32%. We did not detect an elevated risk of incomplete attachment (odds ratio [OR], 1.16; 95% CI, 0.61–2.20), risk of rebubbling (OR, 1.25; 95% CI, 0.47–3.31) or larger areas of graft detachment in non-aligned grafts compared to aligned grafts.

Conclusion
Rotational alignment was not strongly associated with the risk of incomplete graft attachment, although modestly elevated risks cannot be ruled out. Efforts are needed to reduce the need for rebubbling after DMEK and to identify modifiable risk factors for graft detachment

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Acta ophthalmologica. - 99, 8 (2021) , e1334-e1339, ISSN: 1755-3768

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2021
• Creator: Fritz, Marianne ; Grewing, Viviane Caroline ; Gruber, Markus ; Wagner, Helena ; Zander, Daniel ; Lapp, Thabo ; Lang, Stefan Johann ; Heinzelmann-Mink, Sonja Ute ; Maier, Philip ; Reinhard, Thomas ; Wacker, Katrin

Further information

• DOI: 10.1111/aos.14849
• URN: urn:nbn:de:bsz:25-freidok-1944377
• Rights: Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:55 PM CET