Hierarchical TAF1-dependent co-translational assembly of the basal transcription factor TFIID

Abstract: Large heteromeric multiprotein complexes play pivotal roles at every step of gene expression in eukaryotic cells. Among them, the 20-subunit basal transcription factor TFIID nucleates the RNA polymerase II preinitiation complex at gene promoters. Here, by combining systematic RNA-immunoprecipitation (RIP) experiments, single-molecule imaging, proteomics and structure–function analyses, we show that human TFIID biogenesis occurs co-translationally. We discovered that all protein heterodimerization steps happen during protein synthesis. We identify TAF1—the largest protein in the complex—as a critical factor for TFIID assembly. TAF1 acts as a flexible scaffold that drives the co-translational recruitment of TFIID submodules preassembled in the cytoplasm. Altogether, our data suggest a multistep hierarchical model for TFIID biogenesis that culminates with the co-translational assembly of the complex onto the nascent TAF1 polypeptide. We envision that this assembly strategy could be shared with other large heteromeric protein complexes

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Nature structural & molecular biology. - 30, 8 (2023) , 1141-1152, ISSN: 1545-9985

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2023
• Creator: Bernardini, Andrea ; Mukherjee, Pooja ; Scheer, Elisabeth ; Kamenova, Ivanka ; Antonova, Simona V. ; Mendoza Sanchez, Paulina Karen ; Yayli, Gizem ; Morlet, Bastien ; Timmers, Marc ; Tora, László

Further information

• DOI: 10.1038/s41594-023-01026-3
• URN: urn:nbn:de:bsz:25-freidok-2382536
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:46 AM CEST