The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma
Abstract: Melanoma tumors are highly metastatic partly due to the ability of melanoma cells to transition between invasive and proliferative states. However, the mechanisms underlying this plasticity are still not fully understood. To identify new epigenetic regulators of melanoma plasticity, we combined data mining, tumor models, proximity proteomics, and CUT&RUN sequencing. We focus on the druggable family of bromodomain epigenetic readers and identify TRIM28 as a new regulator of melanoma plasticity. We find that TRIM28 promotes the expression of pro‐invasive genes and that TRIM28 controls the balance between invasiveness and growth of melanoma cells. We demonstrate that TRIM28 acts via the transcription factor JUNB that directly regulates the expression of pro‐invasive and pro‐growth genes. Mechanistically, TRIM28 controls the expression of JUNB by negatively regulating its transcriptional elongation by RNA polymerase II. In conclusion, our results demonstrate that a TRIM28–JUNB axis controls the balance between invasiveness and growth in melanoma tumors and suggest that the bromodomain protein TRIM28 could be targeted to reduce tumor spread.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma ; day:07 ; month:11 ; year:2022 ; extent:18
EMBO reports / European Molecular Biology Organization ; (07.11.2022) (gesamt 18)
- Creator
-
Nyberg, William A.
Velasquez‐Pulgarin, Diego A.
He, Tianlin
Sjöstrand, Maria
Pellé, Lucia
Covacu, Ruxandra
Espinosa, Alexander
- DOI
-
10.15252/embr.202254944
- URN
-
urn:nbn:de:101:1-2022110714144306021591
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:31 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Nyberg, William A.
- Velasquez‐Pulgarin, Diego A.
- He, Tianlin
- Sjöstrand, Maria
- Pellé, Lucia
- Covacu, Ruxandra
- Espinosa, Alexander
Other Objects (12)
mTOR inhibitor everolimus reduces invasiveness of melanoma cells
Melanoma Inhibitory Activity (MIA) increases the invasiveness of pancreatic cancer cells
Androgen drives melanoma invasiveness and metastatic spread by inducing tumorigenic fucosylation
Systems analysis identifies miR-29b regulation of invasiveness in melanoma
Selectively Binding a Bromodomain
Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma
The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential
The ACE Inhibitor Lisinopril Stimulates Melanoma Cell Invasiveness by Inducing MMP2 Secretion
Bacterial invasiveness
MMP3 activity rather than cortical stiffness determines NHE1-dependent invasiveness of melanoma cells
Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness
Discovery of a PCAF bromodomain chemical probe
mTOR inhibitor everolimus reduces invasiveness of melanoma cells
Melanoma Inhibitory Activity (MIA) increases the invasiveness of pancreatic cancer cells
Androgen drives melanoma invasiveness and metastatic spread by inducing tumorigenic fucosylation
Systems analysis identifies miR-29b regulation of invasiveness in melanoma
Selectively Binding a Bromodomain
Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma
The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential
The ACE Inhibitor Lisinopril Stimulates Melanoma Cell Invasiveness by Inducing MMP2 Secretion
Bacterial invasiveness
MMP3 activity rather than cortical stiffness determines NHE1-dependent invasiveness of melanoma cells
Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness
Discovery of a PCAF bromodomain chemical probe
mTOR inhibitor everolimus reduces invasiveness of melanoma cells
Melanoma Inhibitory Activity (MIA) increases the invasiveness of pancreatic cancer cells
Androgen drives melanoma invasiveness and metastatic spread by inducing tumorigenic fucosylation
Systems analysis identifies miR-29b regulation of invasiveness in melanoma
Selectively Binding a Bromodomain
Stromal fibroblast growth factor 2 reduces the efficacy of bromodomain inhibitors in uveal melanoma
The mechanosensitive TRPV2 calcium channel promotes human melanoma invasiveness and metastatic potential
The ACE Inhibitor Lisinopril Stimulates Melanoma Cell Invasiveness by Inducing MMP2 Secretion
Bacterial invasiveness
MMP3 activity rather than cortical stiffness determines NHE1-dependent invasiveness of melanoma cells
Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness