Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp.
Abstract: Xylanases constitute an important industrial enzyme, which hydrolyzes the polysaccharide xylan. In this work, a novel Streptomyces strain producing cellulase-free xylanase was isolated from the soil samples collected from the mangrove forest of Kadalundi, Kerala, India. The strain produced unique enzyme, which exhibited optimal activity at pH 9.0 and tolerance up to pH 12.0. Media engineering was carried out to improve the enzyme production, which showed best enzyme production at 30°C, medium pH 9.0 and incubation time of 48 h. Enzyme was highly thermo-tolerant up to 70°C and alkaline tolerant. Partial gene amplification as well as partial purification of enzyme was carried out to characterize the enzyme. The unique features of the enzyme make it an ideal candidate for industrial application for paper and pulp industry.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Identification and characterization of a highly alkaline and thermotolerant novel xylanase from Streptomyces sp. ; volume:68 ; number:6 ; year:2013 ; pages:1022-1027 ; extent:6
Biologia ; 68, Heft 6 (2013), 1022-1027 (gesamt 6)
- Urheber
- DOI
-
10.2478/s11756-013-0248-5
- URN
-
urn:nbn:de:101:1-2409231608501.956509211732
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:27 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Thomas, Leya
- Sindhu, Raveendran
- Pandey, Ashok
Ähnliche Objekte (12)
Highly Thermostable Xylanase Purified from Rhizomucor Miehei NRRL 3169
Modification and application of highly active alkaline pectin lyase
A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization
Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase
Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization
Comparative genomics of Streptomyces avermitilis, Streptomyces cattleya, Streptomyces maritimus and Kitasatospora aureofaciens using a Streptomyces coelicolor microarray system
Hydrolytic Stability of Crosslinked, Highly Alkaline Diallyldimethylammonium Hydroxide Hydrogels
Characterisation of Interspecies Interactions between Streptomyces violaceoruber and Streptomyces aburaviensis
Alkaline Phosphatase and Hypophosphatasia
A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities
Long-term evolution of highly alkaline steel slag drainage waters
Highly Thermostable Xylanase Purified from Rhizomucor Miehei NRRL 3169
Modification and application of highly active alkaline pectin lyase
A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization
Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase
Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization
Comparative genomics of Streptomyces avermitilis, Streptomyces cattleya, Streptomyces maritimus and Kitasatospora aureofaciens using a Streptomyces coelicolor microarray system
Hydrolytic Stability of Crosslinked, Highly Alkaline Diallyldimethylammonium Hydroxide Hydrogels
Characterisation of Interspecies Interactions between Streptomyces violaceoruber and Streptomyces aburaviensis
Alkaline Phosphatase and Hypophosphatasia
A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities
Long-term evolution of highly alkaline steel slag drainage waters
Highly Thermostable Xylanase Purified from Rhizomucor Miehei NRRL 3169
Modification and application of highly active alkaline pectin lyase
A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization
Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase
Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization
Comparative genomics of Streptomyces avermitilis, Streptomyces cattleya, Streptomyces maritimus and Kitasatospora aureofaciens using a Streptomyces coelicolor microarray system
Hydrolytic Stability of Crosslinked, Highly Alkaline Diallyldimethylammonium Hydroxide Hydrogels
Characterisation of Interspecies Interactions between Streptomyces violaceoruber and Streptomyces aburaviensis
Alkaline Phosphatase and Hypophosphatasia
A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities