Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells
Abstract: In this study, we aim to discover the importance of long non-coding RNA cyclin-dependent kinase 6 (CDK6)-AS1 in lipopolysaccharide (LPS)-induced HK-2 cells. We treated the HK-2 cells with LPS and knocked down CDK6-AS1 in HK-2 cells and then analyzed the effects of CDK6-AS1 on the viability of cell, cell apoptosis, the expression of cytokines via MTT, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and qPCR. The results showed that silencing CDK6-AS1 alleviated LPS-induced inhibition of HK-2 cell proliferation, release of IL-1β, IL-8, IL-6, and TNF-α, cell apoptosis, and decrease in mitochondrial membrane potential. In addition, decreasing the level of CDK6-AS1 inhibited the reduction of Bcl-2 levels, the expression of Bax, cleaved caspade-9, and cleaved caspase-3, induced by LPS. In conclusion, lowering CDK6-AS1 level alleviates LPS-induced inflammatory damage in HK-2 cells.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Silencing CDK6-AS1 inhibits LPS-induced inflammatory damage in HK-2 cells ; volume:16 ; number:1 ; year:2021 ; pages:1256-1264 ; extent:9
Open medicine ; 16, Heft 1 (2021), 1256-1264 (gesamt 9)
- Urheber
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Wu, Ling
Zhang, Rui
Lin, Sheng
Lin, Min
Wang, Jing
- DOI
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10.1515/med-2021-0314
- URN
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urn:nbn:de:101:1-2022092014100554289697
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:30 MESZ
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Beteiligte
- Wu, Ling
- Zhang, Rui
- Lin, Sheng
- Lin, Min
- Wang, Jing
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