The ratio of ADP- to TRAP-induced platelet aggregation quantifies P2Y12-dependent platelet inhibition independently of the platelet count

Abstract: Objective This study aimed to assess the association of clinical factors with P2Y 12 -dependent platelet inhibition as monitored by the ratio of ADP- to TRAP-induced platelet aggregation and conventional ADP-induced aggregation, respectively. BackgroundControversial findings to identify and overcome high platelet reactivity (HPR) after coronarystent-implantation and to improve clinical outcome by tailored anti-platelet therapy exist. Monitoring anti-platelet therapy ex vivo underlies several confounding parameters causing that ex vivo platelet aggregation might not reflect in vivoplatelet inhibition.MethodsIn a single centre observational study, multiple electrode aggregometry was performed in whole blood of patients after recent coronary stent-implantation. Relative ADP-induced aggregation (r-ADP-agg) was defined as the ratio of ADP- to TRAP- induced aggregation reflecting the individual degree of P2Y 12-mediated platelet reactivity. ResultsPlatelet aggregation was assessed in 359 patients. Means (±SD) of TRAP-, ADP-induced aggregation and r-ADP-agg were 794±239 AU*min, 297±153 AU*min and 37±14%,respectively. While ADP- and TRAP-induced platelet aggregation correlated significantly with platelet count (ADP: r = 0.302; p<0.001; TRAP: r = 0.509 p <0.001), r-ADP-agg values did not (r = -0.003; p = 0.960). These findings were unaltered in multivariate analysesadjusting for a range of factors potentially influencing platelet aggregation. The presence of an acute coronary syndrome and body weight were found to correlate with both ADP-induced platelet aggregation and r-ADP-agg.ConclusionThe ratio of ADP- to TRAP-induced platelet aggregation quantifies P2Y 12 -dependent plate-let inhibition independently of the platelet count in contrast to conventional ADP-induced aggregation. Furthermore, r-ADP-agg was associated with the presence of an acute coronary syndrome and body weight as well as ADP-induced aggregation. Thus, the r-ADP-aggis a more valid reflecting platelet aggregation and potentially prognosis after coronary stent-implantation in P2Y12-mediated HPR than conventional ADP-induced platelet aggregation

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: PLoS ONE. 11, 2 (2016), e0149053, DOI 10.1371/journal.pone.0149053, issn: 1932-6205 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Classification and Topics

• Keyword: Kardiovaskuläre Krankheit

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2016
• Creator: Olivier, Christoph ; Meyer-Lühmann, Melanie ; Bauer, Hans ; Schnabel, Katharina ; Weik, Patrick ; Zhou, Qianxing ; Bode, Christoph ; Moser, Martin ; Diehl, Philipp
• Contributor: Klinik für Kardiologie und Angiologie I ; Albert-Ludwigs-Universität Freiburg ; Medizinische Fakultät

Further information

• DOI: 10.1371/journal.pone.0149053
• URN: urn:nbn:de:bsz:25-freidok-121164
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:52 PM CET