Fast‐Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body
Abstract: Drosophila melanogaster, the fruit fly, is an excellent model organism for studying dopaminergic mechanisms and simple behaviors, but methods to measure dopamine during behavior are needed. Here, we developed fast‐scan cyclic voltammetry (FSCV) to track in vivo dopamine during sugar feeding. First, we employed acetylcholine stimulation to evaluate the feasibility of in vivo measurements in an awake fly. Next, we tested sugar feeding by placing sucrose solution near the fly proboscis. In the mushroom body medial tip, 1 pmol acetylcholine and sugar feeding released 0.49±0.04 μM and 0.31±0.06 μM dopamine, respectively but sugar‐evoked release lasted longer than with acetylcholine. Administering the dopamine transporter inhibitor nisoxetine or D2 receptor antagonist flupentixol significantly increased sugar‐evoked dopamine. This study develops FSCV to measure behaviorally evoked release in fly, enabling Drosophila studies of neurochemical control of reward, learning, and memory behaviors.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Fast‐Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body ; day:05 ; month:10 ; year:2022 ; extent:1
Angewandte Chemie ; (05.10.2022) (gesamt 1)
- Creator
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Shin, Mimi
Venton, B. Jill
- DOI
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10.1002/ange.202207399
- URN
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urn:nbn:de:101:1-2022100615050000700089
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:38 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Shin, Mimi
- Venton, B. Jill
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Fast‐Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body
Time-dependent assessment of stimulus-evoked regional dopamine release
Simultaneous serotonin and dopamine monitoring across timescales by rapid pulse voltammetry with partial least squares regression
Recent Advances in Voltammetry
Voltammetry coupled with impedance spectroscopy
The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx
Visual Attention in Flies-Dopamine in the Mushroom Bodies Mediates the After-Effect of Cueing
Comparative sulfite assay by voltammetry using Pt electrodes, photometry and titrimetry: Application to cider, vinegar and sugar analysis
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Polarography and voltammetry in trace analysis
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Dopamine release in mushroom bodies of the honey bee (Apis mellifera L.) in response to aversive stimulation
Fast‐Scan Cyclic Voltammetry (FSCV) Reveals Behaviorally Evoked Dopamine Release by Sugar Feeding in the Adult Drosophila Mushroom Body
Time-dependent assessment of stimulus-evoked regional dopamine release
Simultaneous serotonin and dopamine monitoring across timescales by rapid pulse voltammetry with partial least squares regression
Recent Advances in Voltammetry
Voltammetry coupled with impedance spectroscopy
The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx
Visual Attention in Flies-Dopamine in the Mushroom Bodies Mediates the After-Effect of Cueing
Comparative sulfite assay by voltammetry using Pt electrodes, photometry and titrimetry: Application to cider, vinegar and sugar analysis
Mushroom Monstrosities
Polarography and voltammetry in trace analysis
Square wave voltammetry : theory and application