Role of the small protein Mco6 in the mitochondrial sorting and assembly machinery

Abstract: The majority of mitochondrial precursor proteins are imported through the Tom40 β-barrel channel of the translocase of the outer membrane (TOM). The sorting and assembly machinery (SAM) is essential for β-barrel membrane protein insertion into the outer membrane and thus required for the assembly of the TOM complex. Here, we demonstrate that the α-helical outer membrane protein Mco6 co-assembles with the mitochondrial distribution and morphology protein Mdm10 as part of the SAM machinery. MCO6 and MDM10 display a negative genetic interaction, and a mco6-mdm10 yeast double mutant displays reduced levels of the TOM complex. Cells lacking Mco6 affect the levels of Mdm10 and show assembly defects of the TOM complex. Thus, this work uncovers a role of the SAMMco6 complex for the biogenesis of the mitochondrial outer membrane

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch
Notes
Cell reports. - 43, 3 (2024) , 113805, ISSN: 2211-1247

Classification
Medizin, Gesundheit

Event
Veröffentlichung
(where)
Freiburg
(who)
Universität
(when)
2024
Creator
Busto, Jon V.
Ganesan, Iniyan
Mathar, Hannah
Steiert, Conny
Schneider, Eva F.
Straub, Sebastian Philipp
Ellenrieder, Lars
Song, Jiyao
Stiller, Sebastian
Lübbert, Philipp
Chatterjee, Ritwika
Elsaesser, Jana
Melchionda, Laura
Schug, Christina
Brave, Fabian den
Schulte, Uwe
Klecker, Till
Kraft, Claudine
Fakler, Bernd
Becker, Thomas
Wiedemann, Nils

DOI
10.1016/j.celrep.2024.113805
URN
urn:nbn:de:bsz:25-freidok-2442369
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
25.03.2025, 1:48 PM CET

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Associated

Time of origin

  • 2024

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