Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes
Abstract: Ceramidases (CDases) are important in controlling skin barrier integrity by regulating ceramide composition and affording downstream signal molecules. While the functions of epidermal CDases are known, roles of neutral CDases secreted by skin‐residing microbes are undefined. Here, we developed a one‐step fluorogenic substrate, S‐B, for specific detection of bacterial CDase activity and inhibitor screening. We identified a non‐hydrolyzable substrate mimic, C6, as the best hit. Based on C6, we designed a photoaffinity probe, JX‐1, which efficiently detects bacterial CDases. Using JX‐1, we identified endogenous low‐abundance PaCDase in a P. aeruginosa monoculture and in a mixed skin bacteria culture. Harnessing both S‐B and JX‐1, we found that CDase activity positively correlates with the relative abundance of P. aeruginosa and is negatively associated with wound area reduction in clinical diabetic foot ulcer patient samples. Overall, our study demonstrates that bacterial CDases are important regulators of skin ceramides and potentially play a role in wound healing.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes ; day:10 ; month:07 ; year:2023 ; extent:10
Angewandte Chemie / International edition. International edition ; (10.07.2023) (gesamt 10)
- Creator
-
Zou, Jiao Xia
Chua, Wisely
Ser, Zheng
Wang, Shi Mei
Chiang, Grace Shu Hui
Sanmugam, Kavitha
Tan, Boon Yeow
Sobota, Radoslaw M.
Li, Hao
- DOI
-
10.1002/anie.202307553
- URN
-
urn:nbn:de:101:1-2023071115232398148946
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
14.08.2025, 11:04 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zou, Jiao Xia
- Chua, Wisely
- Ser, Zheng
- Wang, Shi Mei
- Chiang, Grace Shu Hui
- Sanmugam, Kavitha
- Tan, Boon Yeow
- Sobota, Radoslaw M.
- Li, Hao
Other Objects (12)
Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes
Reputation, accounting information and debt contracts in Chinese family firms
Differentiation of Bone Marrow Macrophage Precursors from Mouse under the Influence of Interleukin-2, Are Interleukin-2 Activated Macrophage Precursors Natural Killer (NK-) Cells ?
Drug development accelerated by artificial intelligence
On regular solutions to compressible radiation hydrodynamic equations with far field vacuum
Differenzierung von Makrophagen-Vorläufern des Knochenmarks bei der Maus unter dem Einfluss von Interleukin-2 Sind Interleukin-2 aktivierte Makrophagen-Vorläufer natürliche Killer (NK-) Zellen?
The crystal structure of ethyl 2-amino-(4-nitrophenyl)-5-oxo-5,6,7,8-tetrahydro-4 H -chromene-3-carboxylate, C 20 H 22 N 2 O 6
Machine Learning Parameterization of the Multi-scale Kain-Fritsch (MSKF) Convection Scheme and stable simulation coupled in WRF using WRF-ML v1.0
Fully Open Ring Microcavities Based on Metagratings
Adaptive Morley element algorithms for the biharmonic eigenvalue problem
Macrophage precursors derived from murine bone marrow develop into B220+ LAK cells under the influence of Interleukin-2 : A resemblance of these LAK cells to the reported NK-1.1+ CD3– B220+ LAK subset found in spleen cultures : Development of macrophage precursors into B220+ LAK effectors
Searching Lorentz invariance violation from cosmic photon attenuation
Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes
Reputation, accounting information and debt contracts in Chinese family firms
Differentiation of Bone Marrow Macrophage Precursors from Mouse under the Influence of Interleukin-2, Are Interleukin-2 Activated Macrophage Precursors Natural Killer (NK-) Cells ?
Drug development accelerated by artificial intelligence
On regular solutions to compressible radiation hydrodynamic equations with far field vacuum
Differenzierung von Makrophagen-Vorläufern des Knochenmarks bei der Maus unter dem Einfluss von Interleukin-2 Sind Interleukin-2 aktivierte Makrophagen-Vorläufer natürliche Killer (NK-) Zellen?
The crystal structure of ethyl 2-amino-(4-nitrophenyl)-5-oxo-5,6,7,8-tetrahydro-4 H -chromene-3-carboxylate, C 20 H 22 N 2 O 6
Machine Learning Parameterization of the Multi-scale Kain-Fritsch (MSKF) Convection Scheme and stable simulation coupled in WRF using WRF-ML v1.0
Fully Open Ring Microcavities Based on Metagratings
Adaptive Morley element algorithms for the biharmonic eigenvalue problem
Macrophage precursors derived from murine bone marrow develop into B220+ LAK cells under the influence of Interleukin-2 : A resemblance of these LAK cells to the reported NK-1.1+ CD3– B220+ LAK subset found in spleen cultures : Development of macrophage precursors into B220+ LAK effectors
Searching Lorentz invariance violation from cosmic photon attenuation
Detection of Bacterial Neutral Ceramidase in Diabetic Foot Ulcers with an Optimized Substrate and Chemoenzymatic Probes
Reputation, accounting information and debt contracts in Chinese family firms
Differentiation of Bone Marrow Macrophage Precursors from Mouse under the Influence of Interleukin-2, Are Interleukin-2 Activated Macrophage Precursors Natural Killer (NK-) Cells ?
Drug development accelerated by artificial intelligence
On regular solutions to compressible radiation hydrodynamic equations with far field vacuum
Differenzierung von Makrophagen-Vorläufern des Knochenmarks bei der Maus unter dem Einfluss von Interleukin-2 Sind Interleukin-2 aktivierte Makrophagen-Vorläufer natürliche Killer (NK-) Zellen?
The crystal structure of ethyl 2-amino-(4-nitrophenyl)-5-oxo-5,6,7,8-tetrahydro-4 H -chromene-3-carboxylate, C 20 H 22 N 2 O 6
Machine Learning Parameterization of the Multi-scale Kain-Fritsch (MSKF) Convection Scheme and stable simulation coupled in WRF using WRF-ML v1.0
Fully Open Ring Microcavities Based on Metagratings
Adaptive Morley element algorithms for the biharmonic eigenvalue problem
Macrophage precursors derived from murine bone marrow develop into B220+ LAK cells under the influence of Interleukin-2 : A resemblance of these LAK cells to the reported NK-1.1+ CD3– B220+ LAK subset found in spleen cultures : Development of macrophage precursors into B220+ LAK effectors