Enzymatic Insitu Crosslinking Can Improve Hydrogel Stability While Maintaining Matrix Stiffness

Abstract: Next to the choice of a material, the crosslinking characteristics play an important role in the development of hydrogels for applications in the biomedical field. By decreasing crosslinking time or concentration one can reduce the density of the network and hence the stiffness of the hydrogel. However, at the same time in many cases the stability of the matrix is significantly reduced, leading to a rapidly degrading hydrogel structure. In this study, we evaluate and compare two different crosslinking methods while maintaining the material chemistry. The hydrogel system evaluated consisted of oxidized hyaluronic acid and gelatin, and it was crosslinked with microbial transglutaminase. We found that by in situ crosslinking (CL) instead of post CL we can significantly increase the stability of the hydrogel while maintaining the matrix stiffness. Encapsulated embryonic mouse fibroblasts showed an increased viability in in situ crosslinked samples. These findings indicate that it is possible to change hydrogel parameters independently, even if they are otherwise interrelated.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Enzymatic Insitu Crosslinking Can Improve Hydrogel Stability While Maintaining Matrix Stiffness ; volume:9 ; number:33 ; year:2024 ; extent:9
ChemistrySelect ; 9, Heft 33 (2024) (gesamt 9)

Urheber
Kuth, Sonja
Boccaccini, Aldo R.

DOI
10.1002/slct.202401700
URN
urn:nbn:de:101:1-2408281415378.259202562340
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:28 MESZ

Datenpartner

Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.

Beteiligte

Ähnliche Objekte (12)