Meta-lens light-sheet fluorescence microscopy for in vivo imaging
Abstract: Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Meta-lens light-sheet fluorescence microscopy for in vivo imaging ; volume:11 ; number:9 ; year:2022 ; pages:1949-1959 ; extent:11
Nanophotonics ; 11, Heft 9 (2022), 1949-1959 (gesamt 11)
- Urheber
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Luo, Yuan
Tseng, Ming Lun
Vyas, Sunil
Hsieh, Ting-Yu
Wu, Jui-Ching
Chen, Shang-Yang
Peng, Hsiao-Fang
Su, Vin-Cent
Huang, Tzu-Ting
Kuo, Hsin Yu
Chu, Cheng Hung
Chen, Mu Ku
Chen, Jia-Wern
Chen, Yu-Chun
Huang, Kuang-Yuh
Kuan, Chieh-Hsiung
Shi, Xu
Misawa, Hiroaki
Tsai, Din Ping
- DOI
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10.1515/nanoph-2021-0748
- URN
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urn:nbn:de:101:1-2022120813331939145067
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:29 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Luo, Yuan
- Tseng, Ming Lun
- Vyas, Sunil
- Hsieh, Ting-Yu
- Wu, Jui-Ching
- Chen, Shang-Yang
- Peng, Hsiao-Fang
- Su, Vin-Cent
- Huang, Tzu-Ting
- Kuo, Hsin Yu
- Chu, Cheng Hung
- Chen, Mu Ku
- Chen, Jia-Wern
- Chen, Yu-Chun
- Huang, Kuang-Yuh
- Kuan, Chieh-Hsiung
- Shi, Xu
- Misawa, Hiroaki
- Tsai, Din Ping
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