Single Molecule Localization Microscopy for Studying Small Extracellular Vesicles

Abstract: Small extracellular vesicles (sEVs) are 30–200 nm nanovesicles enriched with unique cargoes of nucleic acids, lipids, and proteins. sEVs are released by all cell types and have emerged as a critical mediator of cell‐to‐cell communication. Although many studies have dealt with the role of sEVs in health and disease, the exact mechanism of sEVs biogenesis and uptake remain unexplored due to the lack of suitable imaging technologies. For sEVs functional studies, imaging has long relied on conventional fluorescence microscopy that has only 200–300 nm resolution, thereby generating blurred images. To break this resolution limit, recent developments in super‐resolution microscopy techniques, specifically single‐molecule localization microscopy (SMLM), expanded the understanding of subcellular details at the few nanometer level. SMLM success relies on the use of appropriate fluorophores with excellent blinking properties. In this review, the basic principle of SMLM is highlighted and the state of the art of SMLM use in sEV biology is summarized. Next, how SMLM techniques implemented for cell imaging can be translated to sEV imaging is discussed by applying different labeling strategies to study sEV biogenesis and their biomolecular interaction with the distant recipient cells.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Single Molecule Localization Microscopy for Studying Small Extracellular Vesicles ; day:12 ; month:01 ; year:2023 ; extent:13 ; Small ; (12.01.2023) (gesamt 13)

Beteiligte, Orts- und Zeitangaben

• Urheber: Ghanam, Jamal ; Chetty, Venkatesh Kumar ; Zhu, Xingfu ; Liu, Xiaomin ; Gelléri, Márton ; Barthel, Lennart ; Reinhardt, Dirk ; Cremer, Christoph ; Thakur, Basant Kumar

Weitere Informationen

• DOI: 10.1002/smll.202205030
• URN: urn:nbn:de:101:1-2023011314185430870462
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:31 MESZ