A novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples
Abstract: The interest in extracellular vesicles (EVs) has been increased in recent years due to their potential application in diagnosis and therapy of severe diseases. The versatile fields of application due to the numerous possible cargos and the targeted delivery system make them a promising biopharmaceutical product. However, their broad size range as well as varied surface protein content result in challenges for the purification, characterization, and quantification. In this study a novel method, based on high‐resolution flow cytometry, was examined for the enumeration of EVs in purified as well as crude process samples. In addition to quantification, samples were characterized by dynamic light scattering, zeta potential measurement, and analytical size exclusion chromatography. It has been demonstrated that EVs were successfully enumerated with the novel method, offering great benefits for development and monitoring of EV processes.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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A novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples ; day:14 ; month:01 ; year:2022 ; extent:10
Engineering in life sciences ; (14.01.2022) (gesamt 10)
- Creator
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Kruse, Thomas
Schneider, Samuel
Reger, Lucas Nik
Kampmann, Markus
Reif, Oscar‐Werner
- DOI
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10.1002/elsc.202100149
- URN
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urn:nbn:de:101:1-2022011514341952034384
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:34 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kruse, Thomas
- Schneider, Samuel
- Reger, Lucas Nik
- Kampmann, Markus
- Reif, Oscar‐Werner
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