Microevolution in cyanobacteria: re-sequencing a motile substrain of Synechocystis sp. PCC 6803

Abstract: Synechocystis sp. PCC 6803 is a widely used model cyanobacterium for studying photosynthesis, phototaxis, the production of biofuels and many other aspects. Here we present a re-sequencing study of the genome and seven plasmids of one of the most widely used Synechocystis sp. PCC 6803 substrains, the glucose tolerant and motile Moscow or ‘PCC-M’ strain, revealing considerable evidence for recent microevolution. Seven single nucleotide polymorphisms (SNPs) specifically shared between ‘PCC-M’ and the ‘PCC-N and PCC-P’ substrains indicate that ‘PCC-M’ belongs to the ‘PCC’ group of motile strains. The identified indels and SNPs in ‘PCC-M’ are likely to affect glucose tolerance, motility, phage resistance, certain stress responses as well as functions in the primary metabolism, potentially relevant for the synthesis of alkanes. Three SNPs in intergenic regions could affect the promoter activities of two protein-coding genes and one cis-antisense RNA. Two deletions in ‘PCC-M’ affect parts of clustered regularly interspaced short palindrome repeats-associated spacer-repeat regions on plasmid pSYSA, in one case by an unusual recombination between spacer sequences

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
DNA Research. 19, 6 (2012), 435-448, DOI 10.1093/dnares/dss024, issn: 1340-2838
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Klassifikation
Biowissenschaften, Biologie
Schlagwort
CRISPR/Cas-Methode
Plasmid
Synechocystis

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2012
Urheber
Beteiligte Personen und Organisationen
Albert-Ludwigs-Universität Freiburg
Fakultät für Biologie

DOI
10.1093/dnares/dss024
URN
urn:nbn:de:bsz:25-freidok-118943
Rechteinformation
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Letzte Aktualisierung
25.03.2025, 13:55 MEZ

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Beteiligte

Entstanden

  • 2012

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