Apical bulkheads accumulate as adaptive response to impaired bile flow in liver disease

Abstract: Hepatocytes form bile canaliculi that dynamically respond to the signalling activity of bile acids and bile flow. Little is known about their responses to intraluminal pressure. During embryonic development, hepatocytes assemble apical bulkheads that increase the canalicular resistance to intraluminal pressure. Here, we investigate whether they also protect bile canaliculi against elevated pressure upon impaired bile flow in adult liver. Apical bulkheads accumulate upon bile flow obstruction in mouse models and patients with primary sclerosing cholangitis (PSC). Their loss under these conditions leads to abnormally dilated canaliculi, resembling liver cell rosettes described in other hepatic diseases. 3D reconstruction reveals that these structures are sections of cysts and tubes formed by hepatocytes. Mathematical modelling establishes that they positively correlate with canalicular pressure and occur in early PSC stages. Using primary hepatocytes and 3D organoids, we demonstrate that excessive canalicular pressure causes the loss of apical bulkheads and formation of rosettes. Our results suggest that apical bulkheads are a protective mechanism of hepatocytes against impaired bile flow, highlighting the role of canalicular pressure in liver diseases.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Apical bulkheads accumulate as adaptive response to impaired bile flow in liver disease ; day:31 ; month:07 ; year:2023 ; extent:22 ; EMBO reports / European Molecular Biology Organization ; (31.07.2023) (gesamt 22)

Contributors, Places and Time

• Creator: Mayer, Carlotta ; Nehring, Sophie ; Kücken, Michael ; Repnik, Urska ; Seifert, Sarah ; Sljukic, Aleksandra ; Delpierre, Julien ; Morales‐Navarrete, Hernán ; Hinz, Sebastian ; Brosch, Mario ; Chung, Brian ; Karlsen, Tom ; Huch, Meritxell ; Kalaidzidis, Yannis ; Brusch, Lutz ; Hampe, Jochen ; Schafmayer, Clemens ; Zerial, Marino

Further information

• DOI: 10.15252/embr.202357181
• URN: urn:nbn:de:101:1-2023080115011102145201
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:50 AM CEST