Centrosome function is critical during terminal erythroid differentiation
Abstract: Red blood cells are produced by terminal erythroid differentiation, which involves the dramatic morphological transformation of erythroblasts into enucleated reticulocytes. Microtubules are important for enucleation, but it is not known if the centrosome, a key microtubule‐organizing center, is required as well. Mice lacking the conserved centrosome component, CDK5RAP2, are likely to have defective erythroid differentiation because they develop macrocytic anemia. Here, we show that fetal liver‐derived, CDK5RAP2‐deficient erythroid progenitors generate fewer and larger reticulocytes, hence recapitulating features of macrocytic anemia. In erythroblasts, but not in embryonic fibroblasts, loss of CDK5RAP2 or pharmacological depletion of centrosomes leads to highly aberrant spindle morphologies. Consistent with such cells exiting mitosis without chromosome segregation, tetraploidy is frequent in late‐stage erythroblasts, thereby giving rise to fewer but larger reticulocytes than normal. Our results define a critical role for CDK5RAP2 and centrosomes in spindle formation specifically during blood production. We propose that disruption of centrosome and spindle function could contribute to the emergence of macrocytic anemias, for instance, due to nutritional deficiency or exposure to chemotherapy.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Centrosome function is critical during terminal erythroid differentiation ; day:09 ; month:06 ; year:2022 ; extent:34
The EMBO journal / European Molecular Biology Organization ; (09.06.2022) (gesamt 34)
- Urheber
-
Tátrai, Péter
Gergely, Fanni
- DOI
-
10.15252/embj.2021108739
- URN
-
urn:nbn:de:101:1-2022060915350638708351
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:37 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Tátrai, Péter
- Gergely, Fanni
Ähnliche Objekte (12)
PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells
Fine‐Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation
Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways
Caspase-3 cleaves hnRNP K in erythroid differentiation
Differentiation of human induced pluripotent stem cells into erythroid cells
Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation
miR-1915-3p regulates megakaryocytic and erythroid differentiation by targeting SOCS4
p53 immunohistochemistry discriminates between pure erythroid leukemia and reactive erythroid hyperplasia
![Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells](/assets/placeholder/searchResultMediaNoDigitisedMedia.png)
Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells
Globin gene expression in correlation with G protein-related genes during erythroid differentiation
Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells
PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells
Fine‐Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation
Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways
Caspase-3 cleaves hnRNP K in erythroid differentiation
Differentiation of human induced pluripotent stem cells into erythroid cells
Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation
miR-1915-3p regulates megakaryocytic and erythroid differentiation by targeting SOCS4
p53 immunohistochemistry discriminates between pure erythroid leukemia and reactive erythroid hyperplasia
Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells
Globin gene expression in correlation with G protein-related genes during erythroid differentiation
Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells
PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells
Fine‐Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation
Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways
Caspase-3 cleaves hnRNP K in erythroid differentiation
Differentiation of human induced pluripotent stem cells into erythroid cells
Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation
miR-1915-3p regulates megakaryocytic and erythroid differentiation by targeting SOCS4
p53 immunohistochemistry discriminates between pure erythroid leukemia and reactive erythroid hyperplasia
Involvement of PI3Kγ [PI3Kgamma] in the regulation of erythroid differentiation of K562 cells
Globin gene expression in correlation with G protein-related genes during erythroid differentiation