Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium‐Containing Poly (Methacrylamide) s

Abstract: A highly efficient transfection agent is reported that is based on terpolymer consisting of N‐(2‐hydroxypropyl) methacrylamide (HPMA), N‐(3‐guanidinopropyl) methacrylamide (GPMA), and N‐(2‐indolethyl) methacrylamide monomers (IEMA) by analogy to the amphipathic cell‐penetrating peptides containing tryptophan and arginine residues. The incorporation of the indole‐bearing monomer leads to successful plasmid DNA condensation even at a nitrogen‐to‐phosphate (N/P) ratio of 1. The hydrodynamic diameter of polyplexes is determined to be below 200 nm for all N/P ratios. The transfection studies demonstrate a 200‐fold increase of the transgene expression in comparison to P (HPMA‐co‐GPMA) with the same guanidinium content. This study reveals the strong potential of the indole group as a side‐chain pendant group that can increase the cellular uptake of polymers and the transfection efficiency of the respective polyplexes.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Incorporation of Indole Significantly Improves the Transfection Efficiency of Guanidinium‐Containing Poly (Methacrylamide) s ; volume:41 ; number:6 ; year:2020 ; extent:5 ; Macromolecular rapid communications ; 41, Heft 6 (2020) (gesamt 5)

Contributors, Places and Time

• Creator: Cokca, Ceren ; Zartner, Leon ; Tabujew, Ilja ; Fischer, Dagmar ; Peneva, Kalina

Further information

• DOI: 10.1002/marc.201900668
• URN: urn:nbn:de:101:1-2022062213123479971347
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:31 AM CEST