An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater
Abstract: The valorization of olive mill wastewaters (OMWW), a by‐product of the olive milling, is getting rising attention. Lipophilization of the main phenolic compound 3‐hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant of the promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE was used in a segmented flow setting to allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond β‐glucosidase and the hydrolytic activity of PestE_I208A_L209F_N288A.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater ; day:08 ; month:07 ; year:2022 ; extent:5
Chemie - Ingenieur - Technik ; (08.07.2022) (gesamt 5)
- Urheber
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Terholsen, Henrik
Kaur, Jasmin
Kaloudis, Nikolaos
Staudt, Amanda
Pavlidis, Ioannis V.
Bornscheuer, Uwe T.
- DOI
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10.1002/cite.202200069
- URN
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urn:nbn:de:101:1-2022070915013435148059
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:36 MESZ
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Beteiligte
- Terholsen, Henrik
- Kaur, Jasmin
- Kaloudis, Nikolaos
- Staudt, Amanda
- Pavlidis, Ioannis V.
- Bornscheuer, Uwe T.
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