Allergenicity and IgE Recognition of New Dau c 1 Allergens from Carrot

Scope: Carrot (Daucus carota) allergy is caused by the major carrot allergen Dau c 1, which is a mixture of several isoallergens and variants with sequence identities of >67% or >90%, respectively. However, little is known about the qualitative and quantitative composition of natural Dau c 1. Methods and results: Mass spectrometry of isolated natural Dau c 1 reveals the existence of several yet unknown Dau c 1‐like proteins. The study expresses four Dau c 1‐like proteins in Escherichia coli. Two of the purified proteins, designated Dau c 1.0501 and 1.0601, exhibit sequence identities to Dau c 1.0101 and 1.0401 between 54% and 87%. They possess allergenic potential and are accepted as new isoallergens. One protein, designated as Dau c 1‐like is >50% identical with the new isoallergens but exhibits no allergenicity. Sequence and structural comparisons of this protein with the known Dau c 1 isoallergens offer relevant clues about putative structural IgE epitopes. Conclusion: Identification of new isoallergens and the identification of IgE epitopes may contribute to a more refined component resolved diagnosis and may lay ground for further epitope mapping and personalized targeted treatment approaches of carrot allergy in preclinical and clinical studies.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Allergenicity and IgE Recognition of New Dau c 1 Allergens from Carrot ; day:22 ; month:12 ; year:2022 ; extent:9 ; Molecular nutrition & food research ; (22.12.2022) (gesamt 9)

Contributors, Places and Time

• Creator: Hendrich, Julian M. ; Wangorsch, Andrea ; Rödel, Katharina ; Jacob, Thessa ; Mahler, Vera ; Wöhrl, Birgitta

Further information

• DOI: 10.1002/mnfr.202200421
• URN: urn:nbn:de:101:1-2022122314035253294118
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:22 AM CEST