Protein O-glycosylation analysis
Abstract: This review provides an overview on the methods available for analysis of O-glycosylation. Three major themes are addressed: analysis of released O-glycans including different O-glycan liberation, derivatization, and detection methods; analysis of formerly O-glycosylated peptides yielding information on O-glycan attachment sites; analysis of O-glycopeptides, representing by far the most informative but also most challenging approach for O-glycan analysis. Although there are various techniques available for the identification of O-linked oligosaccharides, the focus here is on MS fragmentation techniques such as collision-induced fragmentation, electron capture dissociation, and electron transfer dissociation. Finally, the O-glycan analytical challenges that need to be met will be discussed.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Protein O-glycosylation analysis ; volume:393 ; number:8 ; year:2012 ; pages:687-708
Biological chemistry ; 393, Heft 8 (2012), 687-708
- Creator
-
Zauner, Gerhild
Kozak, Radoslaw P.
Gardner, Richard A.
Fernandes, Daryl L.
Deelder, André M.
Wuhrer, Manfred
- DOI
-
10.1515/hsz-2012-0144
- URN
-
urn:nbn:de:101:1-2408051712172.683511746752
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
14.08.2025, 10:59 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zauner, Gerhild
- Kozak, Radoslaw P.
- Gardner, Richard A.
- Fernandes, Daryl L.
- Deelder, André M.
- Wuhrer, Manfred
Other Objects (12)
Bioprocessing and protein glycosylation analysis
Human plasma protein N-glycosylation
Epigenetic regulation of protein glycosylation
Optimizing the glycosylation of recombinant proteins
Restoring protein glycosylation with GlycoShape
Diabetes and protein glycosylation : measurement and biologic relevance
Insights into bacterial protein glycosylation in human microbiota
Protein glycosylation in infectious disease pathobiology and treatment
Visualization of Protein-Specific Glycosylation inside Living Cells
Elucidating protein glycosylation mechanisms by combining network-based pathway analysis with prior knowledge
Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)
Intramolecular glycosylation
Bioprocessing and protein glycosylation analysis
Human plasma protein N-glycosylation
Epigenetic regulation of protein glycosylation
Optimizing the glycosylation of recombinant proteins
Restoring protein glycosylation with GlycoShape
Diabetes and protein glycosylation : measurement and biologic relevance
Insights into bacterial protein glycosylation in human microbiota
Protein glycosylation in infectious disease pathobiology and treatment
Visualization of Protein-Specific Glycosylation inside Living Cells
Elucidating protein glycosylation mechanisms by combining network-based pathway analysis with prior knowledge
Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)
Intramolecular glycosylation
Bioprocessing and protein glycosylation analysis
Human plasma protein N-glycosylation
Epigenetic regulation of protein glycosylation
Optimizing the glycosylation of recombinant proteins
Restoring protein glycosylation with GlycoShape
Diabetes and protein glycosylation : measurement and biologic relevance
Insights into bacterial protein glycosylation in human microbiota
Protein glycosylation in infectious disease pathobiology and treatment
Visualization of Protein-Specific Glycosylation inside Living Cells
Elucidating protein glycosylation mechanisms by combining network-based pathway analysis with prior knowledge
Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)