Characterization of the REC114‐MEI4‐IHO1 complex regulating meiotic DNA double‐strand break formation
Abstract: Meiotic recombination is initiated by the formation of DNA double‐strand breaks (DSBs), essential for fertility and genetic diversity. In the mouse, DSBs are formed by the catalytic TOPOVIL complex consisting of SPO11 and TOPOVIBL. To preserve genome integrity, the activity of the TOPOVIL complex is finely controlled by several meiotic factors including REC114, MEI4, and IHO1, but the underlying mechanism is poorly understood. Here, we report that mouse REC114 forms homodimers, that it associates with MEI4 as a 2:1 heterotrimer that further dimerizes, and that IHO1 forms coiled‐coil‐based tetramers. Using AlphaFold2 modeling combined with biochemical characterization, we uncovered the molecular details of these assemblies. Finally, we show that IHO1 directly interacts with the PH domain of REC114 by recognizing the same surface as TOPOVIBL and another meiotic factor ANKRD31. These results provide strong evidence for the existence of a ternary IHO1‐REC114‐MEI4 complex and suggest that REC114 could act as a potential regulatory platform mediating mutually exclusive interactions with several partners.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Characterization of the REC114‐MEI4‐IHO1 complex regulating meiotic DNA double‐strand break formation ; day:11 ; month:07 ; year:2023 ; extent:17
The EMBO journal / European Molecular Biology Organization ; (11.07.2023) (gesamt 17)
- Urheber
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Laroussi, Hamida
Juarez‐Martinez, Ariadna B.
Le Roy, Aline
Boeri Erba, Elisabetta
Gabel, Frank
de Massy, Bernard
Kadlec, Jan
- DOI
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10.15252/embj.2023113866
- URN
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urn:nbn:de:101:1-2023071116300360145391
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:50 MESZ
Datenpartner
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Beteiligte
- Laroussi, Hamida
- Juarez‐Martinez, Ariadna B.
- Le Roy, Aline
- Boeri Erba, Elisabetta
- Gabel, Frank
- de Massy, Bernard
- Kadlec, Jan
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