The tubulogenic effect of aldosterone is attributed to intact binding and intracellular response of the mineralocorticoid receptor
Abstract: Little is known about the extra- and intracellular stimuli inducing renal stem/progenitor cells to develop into three-dimensionally structured tubules. To study this specific development in a controlled environment, we used an advanced culture technique. Embryonic tissue derived from neonatal rabbit kidney was placed in a perfusion culture container at the interface of an artificial interstitium made of a polyester fleece. Culture was carried out in chemically defined Iscove’s Modified Dulbecco’s Medium (IMDM) for 13 days. Development of tubules was histochemically detected on cryosections labeled with Soybean Agglutinin (SBA). The experiments showed that aldosterone exerts a specific tubulogenic effect. Application of aldosterone (1 × 10−7 M) raised numerous SBA-labeled tubules, while in the absence of the steroid hormone the development of tubules was lacking. Specificity of hormone action was analyzed by the use of aldosterone antagonists. Administration of spironolactone (1 × 10−4 M) and canrenoate (1 × 10−5 M) completely inhibited the development of tubules. Finally, disrupting the intracellular molecular complex of the mineralocorticoid receptor (MR) and heat shock proteins by geldanamycin (2 μg/ml) prevented the development of tubules. Our results suggest that the tubulogenic effect induced by aldosterone is attributed to both hormone binding and an undisturbed intracellular response of the MR.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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The tubulogenic effect of aldosterone is attributed to intact binding and intracellular response of the mineralocorticoid receptor ; volume:2 ; number:3 ; year:2007 ; pages:307-325 ; extent:19
Open life sciences ; 2, Heft 3 (2007), 307-325 (gesamt 19)
- Urheber
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Minuth, Will
Denk, Lucia
Hu, Kanghong
Castrop, Hayo
Gomez-Sanchez, Celso
- DOI
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10.2478/s11535-007-0030-1
- URN
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urn:nbn:de:101:1-2409201833480.035864945270
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:27 MESZ
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Beteiligte
- Minuth, Will
- Denk, Lucia
- Hu, Kanghong
- Castrop, Hayo
- Gomez-Sanchez, Celso
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