In vitro study on the effects of exogenic fibrolytic enzymes produced from <italic>Trichoderma longibrachiatum</italic> on ruminal degradation of olive mill waste

Abstract µ L g - 1 µ L g - 1  (high) of dry matter olive mill waste in an air-conditioned room at 26 ∘ C for 12 h before in vitro incubation. For the crude olive cake, this additive at high doses increased degradation of 14 % of cellulose and 8 % of hemicellulose compared with the control at 12 h before the in vitro incubation. Consequently, it increased dry matter solubility and reduced sugars at this period compared to the control. Upon ruminal incubation, the high dose of exogenous fibrolytic enzyme increased the gas production from the immediately soluble fraction and insoluble fraction, the rate of gas production for the insoluble fraction, the dry matter degradability by 26 %, the organic matter degradability by 24 %, the metabolizable energy value by 28 %, and the microbial crude protein production by 24 % compared with the control. For olive leaves, an exogenous fibrolytic enzyme at medium dosage can also hydrolyse the hemicellulose compound, release fewer sugars, and increase dry matter solubility compared with the control at 12 h before the in vitro incubation. Upon in vitro incubation, the medium dose increased the gas production from immediately soluble and insoluble fractions, the rate of gas production for the insoluble fraction, the dry matter degradability by 13 %, the organic matter degradability by 11 %, the metabolizable energy value by 12 %, and the microbial crude protein production by 12 % compared with the control. However, the highest dose altered the gas production from insoluble fractions and decreased microbial crude protein production by 6 % compared with the control. Under the same conditions, an exogenous fibrolytic enzyme applied to extracted olive cake did not produce any effect in the chemical composition and nutritional value. These results showed clearly that effectiveness of exogenous fibrolytic enzyme varied with incubated waste. Increasing the nutritional value of crude olive cake and olive leaves using an exogenous fibrolytic enzyme can encourage breeders to use this waste as feed at a low cost in animal nutrition. This valorization of waste is a good solution to reduce pollution of soils and groundwater caused by throwing out this polluted waste into the environment.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: In vitro study on the effects of exogenic fibrolytic enzymes produced from Trichoderma longibrachiatum on ruminal degradation of olive mill waste ; volume:65 ; number:1 ; year:2022 ; pages:79-88 ; extent:10 ; Archives animal breeding ; 65, Heft 1 (2022), 79-88 (gesamt 10)

Contributors, Places and Time

• Creator: Abid, Khalil ; Jabri, Jihene ; Yaich, Hela ; Malek, Atef ; Rekhis, Jamel ; Kamoun, Mohamed

Further information

• DOI: 10.5194/aab-65-79-2022
• URN: urn:nbn:de:101:1-2022022404211350915992
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:27 AM CEST