Multicolor 3D Orbital Tracking

Abstract: Feedback‐based single‐particle tracking (SPT) is a powerful technique for investigating particle behavior with very high spatiotemporal resolution. The ability to follow different species and their interactions independently adds a new dimension to the information available from SPT. However, only a few approaches have been expanded to multiple colors and no method is currently available that can follow two differently labeled biomolecules in 4 dimensions independently. In this proof‐of‐concept paper, the new modalities available when performing 3D orbital tracking with a second detection channel are demonstrated. First, dual‐color tracking experiments are described studying independently diffusing particles of different types. For interacting particles where their motion is correlated, a second modality is implemented where a particle is tracked in one channel and the position of the second fluorescence species is monitored in the other channel. As a third modality, 3D orbital tracking is performed in one channel while monitoring its spectral signature in a second channel. This last modality is used to successfully readout accurate Förster Resonance Energy Transfer (FRET) values over time while tracking a mobile particle.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Multicolor 3D Orbital Tracking ; day:29 ; month:01 ; year:2023 ; extent:16 ; Small ; (29.01.2023) (gesamt 16)

Contributors, Places and Time

• Creator: Mieskes, Frank ; Ploetz, Evelyn ; Wehnekamp, Fabian ; Rat, Virgile ; Lamb, Don C.

Further information

• DOI: 10.1002/smll.202204726
• URN: urn:nbn:de:101:1-2023012914113744027424
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:25 AM CEST