Opsonization-independent antigen-specific recognition by myeloid phagocytes expressing monoclonal antibodies

Abstract: This report demonstrates a novel class of innate immune cells designated “variable immunoreceptor–expressing myeloids” (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell surface signatures and regularly circulate in the blood of healthy individuals. Single-cell data reveal clonal expansion of circulating B-VIREMs as a dynamic response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport to the cell surface. A first cloned B-VIREM–derived antibody (Vab1) specifically binds stomatin, a ubiquitous scaffold protein that is strictly expressed intracellularly, allowing Vab1-bearing macrophages to phagocytose cell debris without requiring prior opsonization. Our results suggest important antigen-specific tissue maintenance functionalities in these innate immune cells

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Science advances. - 9, 35 (2023) , eadg1812, ISSN: 2375-2548

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2023
• Creator: Neumaier, Michael ; Giesler, Sophie ; Ast, Volker ; Römer, Mathis ; Voß, Timo-Daniel ; Reinz, Eileen ; Costina, Victor ; Schmelz, Martin ; Nürnberg, Elina ; Nittka, Stefanie ; Leppä, Aino-Maija ; Rudolf, Rüdiger ; Trumpp, Andreas ; Fuchs, Tina

Further information

• DOI: 10.1126/sciadv.adg1812
• URN: urn:nbn:de:bsz:25-freidok-2389450
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:47 PM CET