Epitopes Displayed in a Cyclic Peptide Scaffold Bind SARS‐COV‐2 Antibodies

Abstract: The SARS‐CoV‐2 virus that causes COVID‐19 is a global health issue. The spread of the virus has resulted in seven million deaths to date. The emergence of new viral strains highlights the importance of continuous surveillance of the SARS‐CoV‐2 virus by using timely and accurate diagnostic tools. Here, we used a stable cyclic peptide scaffolds to present antigenic sequences derived from the spike protein that are reactive to SARS‐CoV‐2 antibodies. Using peptide sequences from different domains of SARS‐CoV‐2 spike proteins, we grafted epitopes on the peptide scaffold sunflower trypsin inhibitor 1 (SFTI‐1). These scaffold peptides were then used to develop an ELISA to detect SARS‐CoV‐2 antibodies in serum. We show that displaying epitopes on the scaffold improves reactivity overall. One of the scaffold peptides (S2_1146‐1161_c) has reactivity equal to that of commercial assays, and shows diagnostic potential.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Epitopes Displayed in a Cyclic Peptide Scaffold Bind SARS‐COV‐2 Antibodies ; day:12 ; month:07 ; year:2023 ; extent:10 ; ChemBioChem ; (12.07.2023) (gesamt 10)

Contributors, Places and Time

• Creator: Eriksson, Camilla ; Gunasekera, Sunithi ; Muhammad, Taj ; Zhang, Mingshu ; Laurén, Ida ; Mangsbo, Sara M. ; Lord, Martin ; Göransson, Ulf

Further information

• DOI: 10.1002/cbic.202300103
• URN: urn:nbn:de:101:1-2023071215324757736648
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:52 AM CEST