Optimised Synthesis of the Bacterial Magic Spot (p) ppGpp Chemosensor PyDPA

Abstract: Guanosine penta‐ or tetraphosphate (pppGpp or ppGpp, respectively) is a nucleotide signalling molecule with a marked effect on bacterial physiology during stress. Its accumulation slows down cell metabolism and replication, supposedly leading to the formation of the antibiotic‐tolerant persister phenotype. A specifically tailored fluorescent chemosensor, PyDPA, allows the detection of (p) ppGpp in solution with high selectivity, relative to that of other nucleotides. Herein, an optimised synthetic approach is presented that improves the overall yield from 9 to 67 % over 7 steps. The simplicity and robustness of this approach will allow groups investigating the many facets of (p) ppGpp easy access to this probe.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Optimised Synthesis of the Bacterial Magic Spot (p) ppGpp Chemosensor PyDPA ; volume:20 ; number:13 ; year:2019 ; pages:1717-1721 ; extent:5 ; ChemBioChem ; 20, Heft 13 (2019), 1717-1721 (gesamt 5)

Contributors, Places and Time

• Creator: Conti, Gabriele ; Minneci, Marco ; Sattin, Sara

Further information

• DOI: 10.1002/cbic.201900013
• URN: urn:nbn:de:101:1-2022080108030859739246
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:28 AM CEST