Technologies for single-cell isolation

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Abstract: The handling of single cells is of great importance in applications such as cell line development or single-cell analysis, e.g., for cancer research or for emerging diagnostic methods. This review provides an overview of technologies that are currently used or in development to isolate single cells for subsequent single-cell analysis. Data from a dedicated online market survey conducted to identify the most relevant technologies, presented here for the first time, shows that FACS (fluorescence activated cell sorting) respectively Flow cytometry (33% usage), laser microdissection (17%), manual cell picking (17%), random seeding/dilution (15%), and microfluidics/lab-on-a-chip devices (12%) are currently the most frequently used technologies. These most prominent technologies are described in detail and key performance factors are discussed. The survey data indicates a further increasing interest in single-cell isolation tools for the coming years. Additionally, a worldwide patent search was performed to screen for emerging technologies that might become relevant in the future. In total 179 patents were found, out of which 25 were evaluated by screening the title and abstract to be relevant to the field

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
International journal of molecular sciences. 16, 8 (2015), 16897-16919, DOI 10.3390/ijms160816897, issn: 1422-0067
IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Schlagwort
Durchflusscytometrie
Mikrofluidik
Immunologie
Immunsystem
Lymphozyt
Leukozyt
Immunität
Resistenz
Immunchemie

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2015
Urheber
Beteiligte Personen und Organisationen

DOI
10.3390/ijms160816897
URN
urn:nbn:de:bsz:25-freidok-120790
Rechteinformation
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Letzte Aktualisierung
25.03.2025, 13:53 MEZ

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  • 2015

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