Transcriptional Response of White Adipose Tissue to Withdrawal of Vitamin B3

Scope: Distinct markers for mild vitamin B3 deficiency are lacking. To identify these, the molecular responses of white adipose tissue (WAT) to vitamin B3 withdrawal are examined. Methods and results: A dietary intervention is performed in male C57BL/6JRccHsd mice, in which a diet without nicotinamide riboside (NR) is compared to a diet with NR at the recommended vitamin B3 level. Both diets contain low but adequate level of tryptophan. Metabolic flexibility and systemic glucose tolerance are analyzed and global transcriptomics, qRT‐PCR, and histology of epididymal WAT (eWAT) are performed. A decreased insulin sensitivity and a shift from carbohydrate to fatty acid oxidation in response to vitamin B3 withdrawal are observed. This is consistent with molecular changes in eWAT, including an activated MEK/ERK signaling, a lowering of glucose utilization markers, and an increase in makers of fatty acid catabolism, possibly related to the consistent lower expression of mitochondrial electron transport complexes. The synthesis pathway of tetrahydropteridine (BH4), an essential cofactor for neurotransmitter synthesis, is transcriptionally activated. Genes marking these processes are technically validated. Conclusion: The downregulation of Anp32a, Tnk2 and the upregulation of Mapk1, Map2k1, Qdpr, Mthfs, and Mthfsl are proposed as a WAT transcriptional signature marker for mild vitamin B3 deficiency.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Transcriptional Response of White Adipose Tissue to Withdrawal of Vitamin B3 ; volume:63 ; number:13 ; year:2019 ; extent:12 ; Molecular nutrition & food research ; 63, Heft 13 (2019) (gesamt 12)

Contributors, Places and Time

• Creator: Shi, Wenbiao ; Hegeman, Maria A. ; Doncheva, Atanaska ; van der Stelt, Inge ; Bekkenkamp‐Grovenstein, Melissa ; van Schothorst, Evert M. ; Brenner, Charles ; de Boer, Vincent C. J. ; Keijer, Jaap

Further information

• DOI: 10.1002/mnfr.201801100
• URN: urn:nbn:de:101:1-2022072008151798079737
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:33 AM CEST