Single‐Cell Sequencing Reveals Functional Alterations in Tuberculosis
Abstract: Despite its importance, the functional heterogeneity surrounding the dynamics of interactions between mycobacterium tuberculosis and human immune cells in determining host immune strength and tuberculosis (TB) outcomes, remains far from understood. This work now describes the development of a new technological platform to elucidate the immune function differences in individuals with TB, integrating single‐cell RNA sequencing and cell surface antibody sequencing to provide both genomic and phenotypic information from the same samples. Single‐cell analysis of 23 990 peripheral blood mononuclear cells from a new cohort of primary TB patients and healthy controls enables to not only show four distinct immune phenotypes (TB, myeloid, and natural killer (NK) cells), but also determine the dynamic changes in cell population abundance, gene expression, developmental trajectory, transcriptomic regulation, and cell–cell signaling. In doing so, TB‐related changes in immune cell functions demonstrate that the immune response is mediated through host T cells, myeloid cells, and NK cells, with TB patients showing decreased naive, cytotoxicity, and memory functions of T cells, rather than their immunoregulatory function. The platform also has the potential to identify new targets for immunotherapeutic treatment strategies to restore T cells from dysfunctional or exhausted states.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Single‐Cell Sequencing Reveals Functional Alterations in Tuberculosis ; day:08 ; month:01 ; year:2024 ; extent:12
Advanced science ; (08.01.2024) (gesamt 12)
- Creator
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Lyu, Mengyuan
Xu, Gaolian
Zhou, Jian
Reboud, Julien
Wang, Yili
Lai, Hongli
Chen, Yi
Zhou, Yanbing
Zhu, Guiying
Cooper, Jonathan M.
Ying, Binwu
- DOI
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10.1002/advs.202305592
- URN
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urn:nbn:de:101:1-2024010914255914955806
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Lyu, Mengyuan
- Xu, Gaolian
- Zhou, Jian
- Reboud, Julien
- Wang, Yili
- Lai, Hongli
- Chen, Yi
- Zhou, Yanbing
- Zhu, Guiying
- Cooper, Jonathan M.
- Ying, Binwu
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Crystal structure of dimethyl (R)-2-(3-(1-phenylethyl) thioureido)-(1,1′-biphenyl)-4,4′-dicarboxylate, C 25 H 24 N 2 O 4 S
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A unified approach to holomorphic anomaly equations and quantum spectral curves
Sudakov suppression of the Balitsky-Kovchegov kernel
On Itzykson-Zuber Ansatz
Light‐Induced Quantum Anomalous Hall Effect on the 2D Surfaces of 3D Topological Insulators
Existence of solutions for a class of Kirchhoff-type equations with indefinite potential
On fermionic representation of the framed topological vertex
Zur Axiomatik der ebenen Geometrie von Lie über einem Körper
The evolution of the small x gluon TMD
Functional Silver‐Loaded Porous Composite Scaffold for Bone Tissue Bacterial Infection
A Set of Chinese Folk Songs : Vol. 1-3
Crystal structure of dimethyl (R)-2-(3-(1-phenylethyl) thioureido)-(1,1′-biphenyl)-4,4′-dicarboxylate, C 25 H 24 N 2 O 4 S
Modeling the Solvolysis of Composite Materials of Wind Turbine Blades
A unified approach to holomorphic anomaly equations and quantum spectral curves
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On Itzykson-Zuber Ansatz
Light‐Induced Quantum Anomalous Hall Effect on the 2D Surfaces of 3D Topological Insulators
Existence of solutions for a class of Kirchhoff-type equations with indefinite potential