Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon
Scope: Colon metabolomes associated with high‐fat (H) versus energy‐restricted (E) diets in early colorectal cancer (CRC) models have never been directly compared. The objectives of this study are to elucidate metabolites associated with diet, aberrant crypt foci (ACF), and diet:ACF interaction, using a lifetime murine model. Methods and results: Three‐week‐old mice consumed control (C), E, or H initiation diets for 18 weeks. ACF formation is initiated weeks 16–21 with azoxymethane injections, followed by progression diet crossover (to C, E, or H) through week 60. Colon extracts are analyzed using ultra‐high‐performance liquid chromatography‐high resolution mass spectrometry (UHPLC‐HRMS). Metabolites associated with diet, ACF, or diet:ACF are determined using regression models (FDR‐adjusted p‐value <0.05). No metabolites are significantly associated with initiation diets, but concentrations of acylcarnitines and phospholipids are associated with C, E, and H progression diets. Purines, taurine, and phospholipids are associated with ACF presence. No significant associations between metabolites and diet:ACF interaction are observed. Conclusions: These results suggest that recent, rather than early‐life, diet is more closely associated with the colon metabolome, particularly lipid metabolism. Results from this study also provide candidate biomarkers of early CRC development and provide support for the importance of early diet on influencing pre‐CRC risk.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Dietary Energy Intake and Presence of Aberrant Crypt Foci Are Associated with Phospholipid, Purine, and Taurine Metabolite Abundances in C57BL/6N Mouse Colon ; volume:66 ; number:20 ; year:2022 ; extent:12
Molecular nutrition & food research ; 66, Heft 20 (2022) (gesamt 12)
- Creator
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Chatelaine, Haley A.
Ramazani, Cynthia A.
Spencer, Kyle
Olivo‐Marston, Susan
Bailey, Michael T.
McElroy, Joseph
Hatzakis, Emmanuel
Mathé, Ewy A.
Kopec, Rachel E.
- DOI
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10.1002/mnfr.202200180
- URN
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urn:nbn:de:101:1-2022102115205523211005
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Chatelaine, Haley A.
- Ramazani, Cynthia A.
- Spencer, Kyle
- Olivo‐Marston, Susan
- Bailey, Michael T.
- McElroy, Joseph
- Hatzakis, Emmanuel
- Mathé, Ewy A.
- Kopec, Rachel E.
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Detection of aberrant crypt foci with image-enhanced endoscopy
Molecular detection of CD44v6 on aberrant crypt foci by confocal laser endoscopy
Occurrence of colorectal aberrant crypt foci depending on age and dietary patterns of patients
Mismatch repair-deficient crypt foci in Lynch syndrome : molecular alterations and association with clinical parameters
Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1
Eicosapentaenoic acid (EPA) efficacy for colorectal aberrant crypt foci (ACF): a double-blind randomized controlled trial
Magnification endoscopy to detect dysplastic aberrant crypt foci in Mexican patients with high risk for colorectal cancer
Communicating CryptPad
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Rectal aberrant crypt foci (ACF) as a predictor of benign and malignant neoplastic lesions in the large intestine
Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat
Tracks from the Crypt
Detection of aberrant crypt foci with image-enhanced endoscopy
Molecular detection of CD44v6 on aberrant crypt foci by confocal laser endoscopy
Occurrence of colorectal aberrant crypt foci depending on age and dietary patterns of patients
Mismatch repair-deficient crypt foci in Lynch syndrome : molecular alterations and association with clinical parameters
Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1
Eicosapentaenoic acid (EPA) efficacy for colorectal aberrant crypt foci (ACF): a double-blind randomized controlled trial
Magnification endoscopy to detect dysplastic aberrant crypt foci in Mexican patients with high risk for colorectal cancer
Communicating CryptPad
Taurine 6
Rectal aberrant crypt foci (ACF) as a predictor of benign and malignant neoplastic lesions in the large intestine
Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat