Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway
Abstract: This study investigated whether artemisinin (ART) exerts a neuroprotective effect against cerebral ischemia/reperfusion (I/R) injury. Hypoxia-glucose deprivation and reoxygenation (OGD/R) of SH-SY5Y cells were used as the I/R injury model in vitro. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and lactate dehydrogenase (LDH) release was measured. Cell apoptosis and apoptosis-associated protein expression were determined via flow cytometry and western blotting, respectively. The levels of glutathione peroxidase, superoxide dismutase, catalase, and malondialdehyde were determined. The secretion of tumor necrosis factor-α and interleukin-1β was measured using ELISA. The activation of the nuclear factor kappa B (NF-κB) pathway was also determined. The indicated ART concentrations (0, 25, 50, 75, and 100 μM) had no significant effect on SH-SY5Y cell viability and LDH activity. ART promoted cell viability, reduced cell apoptosis, repressed cellular inflammation, and inhibited cellular oxidative stress and NF-κB signaling pathway in OGD/R-induced SH-SY5Y cells. In addition, all the protective effects of ART on OGD/R-induced SH-SY5Y cell injury were significantly reversed by an NF-κB agonist. In conclusion, ART protects neurons from OGD/R-induced damage in vitro by inhibiting the NF-κB signaling pathway. These results suggest that ART may be a potential agent for the treatment of cerebral I/R injury.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Artemisinin protects against cerebral ischemia and reperfusion injury via inhibiting the NF-κB pathway ; volume:17 ; number:1 ; year:2022 ; pages:871-881 ; extent:11
Open medicine ; 17, Heft 1 (2022), 871-881 (gesamt 11)
- Creator
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Ji, Hui
Jin, Haifeng
Li, Guangwei
Jin, Li
Ren, Xiaoxu
Lv, Ying
Wang, Yuchun
- DOI
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10.1515/med-2022-0435
- URN
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urn:nbn:de:101:1-2022071516491410910305
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:26 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Ji, Hui
- Jin, Haifeng
- Li, Guangwei
- Jin, Li
- Ren, Xiaoxu
- Lv, Ying
- Wang, Yuchun
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