Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

to related objects

Abstract: Oligomerization and glycosylation modulate therapeutic glycoprotein stability and efficacy. The interplay between these two critical attributes on therapeutic glycoproteins, is however often hard to define. Here, we present a native top‐down mass spectrometry (MS) approach to assess the glycosylation status of therapeutic cytokine and hormone assemblies and relate interfacial glycan occupancy to complex stability. We found that interfacial O‐glycan stabilizes tumor necrosis factor‐α trimer. On the contrary, interferon‐β1a dimerization is independent of glycosylation. Moreover, we discovered a unique distribution of N‐glycans on the follicle‐stimulating hormone α subunit. We found that the interfacial N‐glycan, at Asn52 of the α subunit, interacts extensively with the β subunit to regulate the dimer assembly. Overall, we have exemplified a method to link glycosylation with assembly status, for cytokines and hormones, critical for informing optimal stability and bioavailability.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies ; day:10 ; month:11 ; year:2022 ; extent:1
Angewandte Chemie ; (10.11.2022) (gesamt 1)

Creator: Wu, Di
Robinson, Carol V.

DOI: 10.1002/ange.202213170

URN: urn:nbn:de:101:1-2022111114074981415701

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:28 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Wu, Di
Robinson, Carol V.

Other Objects (12)

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Hexachloroethane

Hexachloroethane

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Tidal resonance in the Gulf of Thailand

Tidal resonance in the Gulf of Thailand

An investigation on space debris of unknown origin using proper elements and neural networks

An investigation on space debris of unknown origin using proper elements and neural networks

Cutaneous manifestations of autoinflammatory diseases

Cutaneous manifestations of autoinflammatory diseases

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Hexachloroethane

Hexachloroethane

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Tidal resonance in the Gulf of Thailand

Tidal resonance in the Gulf of Thailand

An investigation on space debris of unknown origin using proper elements and neural networks

An investigation on space debris of unknown origin using proper elements and neural networks

Cutaneous manifestations of autoinflammatory diseases

Cutaneous manifestations of autoinflammatory diseases

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Native Top‐Down Mass Spectrometry Reveals a Role for Interfacial Glycans on Therapeutic Cytokine and Hormone Assemblies

Hexachloroethane

Hexachloroethane

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Consistent thermodynamics and topological classes for the four-dimensional Lorentzian charged Taub-NUT spacetimes

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Crystal structure of 1,3-diisopropyl-4,5-dimethylimidazol-2-ylidene- N, N ′-bis (1,3-bis (2,6-diisopropylphenyl)-1,3-dihydro-2 H -1,3,2-diazaborol-2-yl)- l 2 -germenediamine, C 63 H 94 B 2 GeN 8

Tidal resonance in the Gulf of Thailand

Tidal resonance in the Gulf of Thailand

An investigation on space debris of unknown origin using proper elements and neural networks

An investigation on space debris of unknown origin using proper elements and neural networks

Cutaneous manifestations of autoinflammatory diseases

Cutaneous manifestations of autoinflammatory diseases

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Designed Mutations Alter the Binding Pathways of an Intrinsically Disordered Protein

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Multimodel biometrics Fusion based on FAR and FRR using Triangular Norm

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Detection of melanin influence on skin samples based on Raman spectroscopy and optical coherence tomography dual‐modal approach

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Promoting sustainable agroforestry development: a systematic literature review on the Rice-Fish-Duck-Forest system

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Ultra-spinning Chow’s black holes in six-dimensional gauged supergravity and their properties

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.