Impact of Viscosity on Human Hepatoma Spheroids in Soft Core–Shell Microcapsules
Abstract: The extracellular environment regulates the structures and functions of cells, from the molecular to the tissue level. However, the underlying mechanisms influencing the organization and adaptation of cancer in three‐dimensional (3D) environments are not yet fully understood. In this study, the influence of the viscosity of the environment is investigated on the mechanical adaptability of human hepatoma cell (HepG2) spheroids in vitro, using 3D microcapsule reactors formed with droplet‐based microfluidics. To mimic the environment with different mechanical properties, HepG2 cells are encapsulated in alginate core–shell reservoirs (i.e., microcapsules) with different core viscosities tuned by incorporating carboxymethylcellulose. The significant changes in cell and spheroid distribution, proliferation, and cytoskeleton are observed and quantified. Importantly, changes in the expression and distribution of F‐actin and keratin 8 indicate the relation between spheroid stiffness and viscosity of the surrounding medium. The increase of F‐actin levels in the viscous medium can indicate an enhanced ability of tumor cells to traverse dense tissue. These results demonstrate the ability of cancer cells to dynamically adapt to the changes in extracellular viscosity, which is an important physical cue regulating tumor development, and thus of relevance in cancer biology.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Impact of Viscosity on Human Hepatoma Spheroids in Soft Core–Shell Microcapsules ; day:25 ; month:01 ; year:2024 ; extent:10
Advanced healthcare materials ; (25.01.2024) (gesamt 10)
- Creator
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Peng, Xuan
Janićijević, Željko
Lemm, Sandy
Hauser, Sandra
Knobel, Michael
Pietzsch, Jens
Bachmann, Michael
Baraban, Larysa
- DOI
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10.1002/adhm.202302609
- URN
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urn:nbn:de:101:1-2024012514362901188080
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Peng, Xuan
- Janićijević, Željko
- Lemm, Sandy
- Hauser, Sandra
- Knobel, Michael
- Pietzsch, Jens
- Bachmann, Michael
- Baraban, Larysa
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