Chemical Imaging of RNA‐Tau Amyloid Fibrils at the Nanoscale Using Tip‐Enhanced Raman Spectroscopy
Abstract: In the presence of cofactors, tau protein can form amyloid deposits in the brain which are implicated in many neurodegenerative disorders. Heparin, lipids, and RNA are used to recreate tau aggregates in vitro from recombinant protein. However, the mechanism of interaction of these cofactors and the interactions between cofactors and tau are poorly understood. Herein, we use tip‐enhanced Raman spectroscopy (TERS) to visualize the spatial distribution of adenine, protein secondary structure, and amino acids (arginine, lysine and histidine) in single polyadenosine (polyA)‐induced tau fibrils with nanoscale spatial resolution (<10–20 nm). Based on reference unenhanced and surface‐enhanced Raman spectra, we show that the polyA anionic cofactor is incorporated in the fibril structure and seems to be superficial to the β‐sheet core, but nonetheless enveloped within the random‐coiled fuzzy coat. TERS images also prove the colocalization of positively charged arginine, lysine, and histidine amino acids and negatively charged polyA, which constitutes an important step forward to better comprehend the action of RNA cofactors in the mechanism of formation of toxic tau fibrils. TERS appears as a powerful technique for the identification of cofactors in individual tau fibrils and their mode of interaction.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Chemical Imaging of RNA‐Tau Amyloid Fibrils at the Nanoscale Using Tip‐Enhanced Raman Spectroscopy ; day:13 ; month:11 ; year:2023 ; extent:7
Angewandte Chemie ; (13.11.2023) (gesamt 7)
- Urheber
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Cooney, Gary Sean
Talaga, David
Ury‐Thiery, Vicky
Fichou, Yann
Huang, Yuhan
Lecomte, Sophie
Bonhommeau, Sebastien
- DOI
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10.1002/ange.202314369
- URN
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urn:nbn:de:101:1-2023111414033376230934
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:52 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Cooney, Gary Sean
- Talaga, David
- Ury‐Thiery, Vicky
- Fichou, Yann
- Huang, Yuhan
- Lecomte, Sophie
- Bonhommeau, Sebastien
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