Programmable and Reversible Integrin‐Mediated Cell Adhesion Reveals Hysteresis in Actin Kinetics that Alters Subsequent Mechanotransduction
Abstract: Dynamically evolving adhesions between cells and extracellular matrix (ECM) transmit time‐varying signals that control cytoskeletal dynamics and cell fate. Dynamic cell adhesion and ECM stiffness regulate cellular mechanosensing cooperatively, but it has not previously been possible to characterize their individual effects because of challenges with controlling these factors independently. Therefore, a DNA‐driven molecular system is developed wherein the integrin‐binding ligand RGD can be reversibly presented and removed to achieve cyclic cell attachment/detachment on substrates of defined stiffness. Using this culture system, it is discovered that cyclic adhesion accelerates F‐actin kinetics and nuclear mechanosensing in human mesenchymal stem cells (hMSCs), with the result that hysteresis can completely change how hMSCs transduce ECM stiffness. Results are dramatically different from well‐known results for mechanotransduction on static substrates, but are consistent with a mathematical model of F‐actin fragments retaining structure following loss of integrin ligation and participating in subsequent repolymerization. These findings suggest that cyclic integrin‐mediated adhesion alters the mechanosensing of ECM stiffness by hMSCs through transient, hysteretic memory that is stored in F‐actin.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Programmable and Reversible Integrin‐Mediated Cell Adhesion Reveals Hysteresis in Actin Kinetics that Alters Subsequent Mechanotransduction ; day:17 ; month:10 ; year:2023 ; extent:12
Advanced science ; (17.10.2023) (gesamt 12)
- Creator
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Zhang, Zheng
Zhu, Hongyuan
Zhao, Guoqing
Miao, Yunyi
Zhao, Lingzhu
Feng, Jinteng
Zhang, Huan
Miao, Run
Sun, Lin
Gao, Bin
Zhang, Wencheng
Wang, Zheng
Zhang, Jianfang
Zhang, Ying
Guo, Hui
Xu, Feng
Lu, Tian Jian
Genin, Guy M.
Lin, Min
- DOI
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10.1002/advs.202302421
- URN
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urn:nbn:de:101:1-2023101815221083071952
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:55 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zhang, Zheng
- Zhu, Hongyuan
- Zhao, Guoqing
- Miao, Yunyi
- Zhao, Lingzhu
- Feng, Jinteng
- Zhang, Huan
- Miao, Run
- Sun, Lin
- Gao, Bin
- Zhang, Wencheng
- Wang, Zheng
- Zhang, Jianfang
- Zhang, Ying
- Guo, Hui
- Xu, Feng
- Lu, Tian Jian
- Genin, Guy M.
- Lin, Min
Other Objects (12)
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Programmable and Reversible Integrin‐Mediated Cell Adhesion Reveals Hysteresis in Actin Kinetics that Alters Subsequent Mechanotransduction (Adv. Sci. 35/2023)
Ming-ch'u Sun Shen shih-p'u : = Die Rezepte des grossen Sun Lin
Does the Medium-Term Expenditure Framework Work for the Constraint of Debt Scale?
Infinitely Many Solutions for the Nonlinear Schrödinger–Poisson System with Broken Symmetry
On Nodal Solutions of the Nonlinear Choquard Equation
Quantifying the large-scale electrification equilibrium effects in dust storms using field observations at Qingtu Lake Observatory
Chaotic vibration control of a composite cantilever beam
Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation
Stacked multi-electrode design of microbial electrolysis cells for rapid and low-sludge treatment of municipal wastewater
De novo truncating variants of TRIM8 and atypical neuro-renal syndrome: a case report and literature review
Multiple nodal solutions of the Kirchhoff-type problem with a cubic term
Nodal solutions with a prescribed number of nodes for the Kirchhoff-type problem with an asymptotically cubic term
Programmable and Reversible Integrin‐Mediated Cell Adhesion Reveals Hysteresis in Actin Kinetics that Alters Subsequent Mechanotransduction (Adv. Sci. 35/2023)
Ming-ch'u Sun Shen shih-p'u : = Die Rezepte des grossen Sun Lin
Does the Medium-Term Expenditure Framework Work for the Constraint of Debt Scale?
Infinitely Many Solutions for the Nonlinear Schrödinger–Poisson System with Broken Symmetry
On Nodal Solutions of the Nonlinear Choquard Equation
Quantifying the large-scale electrification equilibrium effects in dust storms using field observations at Qingtu Lake Observatory
Chaotic vibration control of a composite cantilever beam
Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation
Stacked multi-electrode design of microbial electrolysis cells for rapid and low-sludge treatment of municipal wastewater
De novo truncating variants of TRIM8 and atypical neuro-renal syndrome: a case report and literature review
Multiple nodal solutions of the Kirchhoff-type problem with a cubic term