Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens

Abstract: Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding. We propose a bi-actuator adaptive lens that simultaneously enables axial scanning and the correction of specimen-induced spherical aberrations with a compact setup. Using the bi-actuator lens in a confocal microscope, we show diffraction-limited axial scanning up to 340 μm deep inside a phantom specimen. The application of this technique to in vivo measurements of zebrafish embryos with reporter-gene-driven fluorescence in a thyroid gland reveals substructures of the thyroid follicles, indicating that the bi-actuator adaptive lens is a meaningful supplement to the existing adaptive optics toolset

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Scientific reports. - 9, 1 (2019) , 9532, ISSN: 2045-2322

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2020
• Creator: Philipp, Katrin ; Lemke, Florian ; Scholz, Stefan ; Wallrabe, Ulrike ; Wapler, Matthias ; Koukourakis, Nektarios ; Czarske, Jürgen
• Contributor: Albert-Ludwigs-Universität Freiburg. Professur für Mikroaktorik

Further information

• DOI: 10.1038/s41598-019-45993-4
• URN: urn:nbn:de:bsz:25-freidok-1543611
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:51 AM CEST